[Histonet] decal solutions

[Joseph Saby]

All-

If you are planning on looking at the bone marrow or are going to be evaluating nuclei in the bone, then I would heartily recommend either using 5% formic acid, or, preferably, a buffered formic acid solution.  The buffer can either be sodium formate or sodium citrate (set to a pH of ~2.2-2.4), but in either case, you can be working with a concentration of 20-25% formice acid and still preserve nuclear detail.  This is not as fast as RDO, but depending on what you want to look at, it can definitely be worth the investment in time!

Joe Saby, BA HT





________________________________
From: Michele Wich <mwich <@t> 7thwavelabs.com>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Tuesday, January 6, 2009 10:00:02 AM
Subject: [Histonet] decal solutions

Can anyone recommend a decalcifying solution that works well for large
animal (dogs, pigs, etc.) bones like femurs and sternums?

Thanks in advance for any input.


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