[Histonet] Question to all my colleagues!

[Tony Henwood]

Rene,

We routinely use xylene for processing. We have a organic solvent recycler so the costs of xylene purchase and disposal are small (though one wonders at the cost of running the recycler!).

Our urgent core biopsies are microwave processed and use iso-propanol (via the Mega TT).

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, 7 January 2009 4:13 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Question to all my colleagues!


First, Happy New Year to all!
 
Second: I am sure you are becoming aware of a growing tendency in tissue processing, namely, the increasing use of iso-propanol, also called isopropylic alcohol or 2-propanol for tissue processing in substitution of both ethanol and specially xylene.
 
Most of those labs using the Peloris tissue processor use iso-propanol and paraffin wax only, but the use is beginning to spread amongst labs that do not work with this instrument.
 
My question is: Would you mind letting me know which laboratories use iso-propanol for tissue processing instead of xylene?
 
I will really appreciate your answer, either directly to me or shared with all in HistoNet. Regards René J.


      
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