[Histonet] IgM monoclonal antibody compatibility

[Patsy Ruegg]

There is definitely a difference in selecting the matching isotype of
secondary for IHC.  Many people learned that lesion back in the day trying
to stain for Hodgkins with CD15 which was IgM, we would get negative results
because the secondary reagents were just recognizing IgG.  It is my
understanding that most of the detection systems commercially sold today
will include more than just IgG to accommodate the use of abs that may be
IgM?  You can use the fact that different abs recognize different Ig subsets
as a tool to do multi labeling in the same tissue, so it can be a good
thing.  If you have one ab that is IgG you could detect it with anti IgG Hrp
for instances, and then if you have a second ab IgM you could detect that
with anti IgM AP for instance and get double labeling even if both abs are
from the same animal species.

Patsy 


Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
email pruegg <@t> ihctech.net
website www.ihctech.net
IHC Resource Group www.ihcrg.org 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of anjan kumar
Sent: Friday, January 02, 2009 9:30 AM
To: triple immunohistochem
Subject: [Histonet] IgM monoclonal antibody compatibility


hi, 
  i wanted to  know if there are any difference in selecting secondary
antibody against IgM compared to secondary antibody for IgG.
as i was using CD24 which is a mouse monoclonal IgM, what kind of secondary
antibody should i select, currently i m hoping that goat anti mouse will
work.
can any one plz suggest me on this.

Regards,
Dr. Anjan Kumar
Junior Research Scientist

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