[Histonet] Differences between WB antibody and IHC antibody

[Swain, Frances L]

You can possibly use it especially if it has been used for IF.  What you need
to do is to get a slide that has a known positive for the antibody, try the
dilution for WB with your standard protocol and see if it works.  If is too
light or too dark you will have to titer it (which should be done in the
first place). If there is no staining you may have to try different things
like enzymatic antigen retrieval or heat etc.  In most cases you can get it
to work to some degree but it is better if you  get one that has been used
with tissue.  The first thing I do is call the company and talk to the
technical personnel to see if they have tested it on tissue samples.  It
takes some work. 

Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Friday, January 02, 2009 1:26 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Differences between WB antibody and IHC antibody

If anyone replies with posting to the list, please forward it.  I've always
wondered the same thing.

On Fri, Jan 2, 2009 at 8:15 AM, TF <tifei <@t> foxmail.com> wrote:

> Hi
> Just wondering whether the antibody for WB (only WB, IP on datasheet) can
> be used for IHC use?
> If not, why?
>
> Can I just increase the concentration for IHC application?
>
> I want to learn more about the underlying production processes.
> Thanks!
>
>
> 2009-01-02
>
>
>
> TF
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