[Histonet] Giemsa Stain

Lee & Peggy Wenk lpwenk <@t> sbcglobal.net
Tue Feb 24 18:30:44 CST 2009

I'd say underfixation with 10% NBF. Try extending the time in formalin,
before going into decal. 

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Caleri,
Sent: Tuesday, February 24, 2009 1:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Giemsa Stain

Our lab was using B-5 to fix our bone marrow specimens, and the resulting
Giemsa stains were very nice...In order to use the pneumatic tube system at
the facility we had to switch to 10% NBF ( we are in the process of choosing
a zinc formalin fixative) and now the Giemsas are a funny turquoise, and
very pale....has anyone ever encountered this? any ideas??
Kathleen Caleri, BS, MLT, HT (ASCP)
Histology Lab Supervisor
Roswell Park Cancer Institute
(716) 845-1329
"the future of mankind lies waiting for those who come to understand their
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