[Histonet] Giemsa Stain
Lee & Peggy Wenk
lpwenk <@t> sbcglobal.net
Tue Feb 24 18:30:44 CST 2009
I'd say underfixation with 10% NBF. Try extending the time in formalin,
before going into decal.
Peggy A. Wenk, HTL(ASCP)SLS
Royal Oak, MI 48073
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Caleri,
Sent: Tuesday, February 24, 2009 1:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Giemsa Stain
Our lab was using B-5 to fix our bone marrow specimens, and the resulting
Giemsa stains were very nice...In order to use the pneumatic tube system at
the facility we had to switch to 10% NBF ( we are in the process of choosing
a zinc formalin fixative) and now the Giemsas are a funny turquoise, and
very pale....has anyone ever encountered this? any ideas??
Kathleen Caleri, BS, MLT, HT (ASCP)
Histology Lab Supervisor
Roswell Park Cancer Institute
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