[Histonet] Giemsa Stain

Caleri, Kathleen Kathleen.Caleri <@t> RoswellPark.org
Tue Feb 24 12:00:02 CST 2009

Our lab was using B-5 to fix our bone marrow specimens, and the
resulting Giemsa stains were very nice...In order to use the pneumatic
tube system at the facility we had to switch to 10% NBF ( we are in the
process of choosing a zinc formalin fixative) and now the Giemsas are a
funny turquoise, and very pale....has anyone ever encountered this? any
Kathleen Caleri, BS, MLT, HT (ASCP)
Histology Lab Supervisor
Roswell Park Cancer Institute
(716) 845-1329
"the future of mankind lies waiting for those who come to understand
their lives and take up their responsibilities to help others"  

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