[Histonet] has anybody done SSEA-1 IHC on mouse embryo?

[Mariya Peneva Dobreva]

Hi everybody,

I need to perform immuno staining for SSEA-1 (stage specific embryonic  
antigen 1) on sectioned early mouse embryo - E6.5. I've tried all  
possible protocols and it never worked - different antigen retrievals,  
different amplification systems, without amplification, different  
incubation times and antibody concentrations, even different  
buffers... I have or too much background, or no signal.
The epitope is on the cell surface, the primary antibody is mouse  
monoclonal, and the embryos are fixed in 4% paraformaldehyde.
Any suggestions are very welcome, I just don't know what else to try!
Thanks!


-- 
Mariya Peneva Dobreva
VIB Department of Molecular and Developmental Genetics (VIB11)
KULeuven Faculty of Medicine, Department of Human Genetics (DME)
Laboratory of Molecular biology (Celgen)
Campus Gasthuisberg, O&N1, Herestraat 49
3000 Leuven, Belgium
Tel.: +32 16 33 00 08
Fax: +32 16 34 59 16

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