[Histonet] has anybody done SSEA-1 IHC on mouse embryo?

Mariya Peneva Dobreva Mariya.Dobreva <@t> med.kuleuven.be
Mon Feb 23 12:41:08 CST 2009

Hi everybody,

I need to perform immuno staining for SSEA-1 (stage specific embryonic  
antigen 1) on sectioned early mouse embryo - E6.5. I've tried all  
possible protocols and it never worked - different antigen retrievals,  
different amplification systems, without amplification, different  
incubation times and antibody concentrations, even different  
buffers... I have or too much background, or no signal.
The epitope is on the cell surface, the primary antibody is mouse  
monoclonal, and the embryos are fixed in 4% paraformaldehyde.
Any suggestions are very welcome, I just don't know what else to try!

Mariya Peneva Dobreva
VIB Department of Molecular and Developmental Genetics (VIB11)
KULeuven Faculty of Medicine, Department of Human Genetics (DME)
Laboratory of Molecular biology (Celgen)
Campus Gasthuisberg, O&N1, Herestraat 49
3000 Leuven, Belgium
Tel.: +32 16 33 00 08
Fax: +32 16 34 59 16

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