[Histonet] how long can slides stay in water or buffer

Mariya Peneva Dobreva Mariya.Dobreva <@t> med.kuleuven.be
Mon Feb 23 12:17:30 CST 2009

Never let them air dry! The staining won't work.
They can stay in water quite some time, like hours. I even think they  
can stay 2-3 days at 4 degrees, but I'm not sure...
With the buffers be careful! They can't stay in the buffers containing  
permeabilyzing agent (like tween20 or triton) longer than what is said  
in the protocol, otherwise you will damage the tissue/loose the  
epitope. There is no problem to keep them in PBS (or TBS) longer.

Quoting Kimberly Tuttle <ktuttle <@t> umm.edu>:

> How long can you leave deparaffinized slides in water or buffer  if   
> you want to do IHC staining? Is leaving them in water or buffer   
> preferable o letting them air dry?  I was taught to never let   
> sections dry out.
> Kimberly C. Tuttle  HT (ASCP)
> Pathology Biorepository and Research Core
> University of Maryland
> Room NBW58, UMMC
> 22 S. Greene St
> Baltimore, MD 21201
> (410) 328-5524
> (410) 328-5508 fax
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Mariya Peneva Dobreva
VIB Department of Molecular and Developmental Genetics (VIB11)
KULeuven Faculty of Medicine, Department of Human Genetics (DME)
Laboratory of Molecular biology (Celgen)
Campus Gasthuisberg, O&N1, Herestraat 49
3000 Leuven, Belgium
Tel.: +32 16 33 00 08
Fax: +32 16 34 59 16

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