[Histonet] FISH-troubleshooting

[Gudrun Lang]

Hi dear listmembers!

What troubleshooting would you suggest for this problem: 
Her2neu FISH with Vysis-standard protocol (30 min  80°C pretreatment
solution, 10 min 37°C 0,05% pepsin)

The staining result is clear CEP-staining in green but no LSI-staining in
red.

 

With the same run, I had an amplified case, that was clearly seen with more
or less bright signals.

 

Hopefully waiting

Gudrun Lang