[Histonet] Half-catalysed PMMA?

Monfils, Paul PMonfils <@t> Lifespan.org
Wed Feb 18 11:49:19 CST 2009

The PMMA embedding protocol we have followed for years calls for infiltration with uncatalysed monomer, then half catalysed monomer, then fully catalysed monomer.  I am wondering if others do something similar.  Specifically, is the intermediate step with half-catalysed monomer really necessary?

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