[Histonet] IF on cell lines

Aprill Watanabe awatanabe <@t> tgen.org
Mon Feb 16 07:13:34 CST 2009

I have a colleague who is trying to do IF on cell culture cell lines.  She
is growing them on cell culture treated chamber slides and fixing with 4%
formaldehyde.  Her problem is that after fixation and a few washes all the
cells are detaching.  Any suggestions?  I think she is going to try 4%
paraformaldehyde first as someone has already switching to that.

Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
main: 602-343-8822
Fax: 602-343-8840
awatanabe <@t> tgen.org

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