[Histonet] freezing paraffin blocks

Rene J Buesa rjbuesa <@t> yahoo.com
Sat Feb 14 10:26:01 CST 2009

Not at all!
René J.

--- On Fri, 2/13/09, Jennifer Anderson <janderson <@t> halozyme.com> wrote:

From: Jennifer Anderson <janderson <@t> halozyme.com>
Subject: [Histonet] freezing paraffin blocks
To: Histonet <@t> lists.utsouthwestern.edu
Date: Friday, February 13, 2009, 5:43 PM

Hi All.


I've just started sectioning Bouin's fixed paraffin embedded
human/mouse/rat/pig skin again, tissues processed in a softer wax than
I'm used to, so I've been putting the blocks in the freezer at -20
to sectioning.  Will such cold deleteriously affect the blocks or


Also, is there any harm in cleaning the water bath with a little xylene
to remove any wax build-up?


Thanks a lot.


Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11404 Sorrento Valley Road

San Diego, CA 92121


janderson <@t> halozyme.com



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