[Histonet] freezing paraffin blocks

[Jennifer Anderson]

Hi All.

 

I've just started sectioning Bouin's fixed paraffin embedded
human/mouse/rat/pig skin again, tissues processed in a softer wax than
I'm used to, so I've been putting the blocks in the freezer at -20 prior
to sectioning.  Will such cold deleteriously affect the blocks or
tissue?

 

Also, is there any harm in cleaning the water bath with a little xylene
to remove any wax build-up?

 

Thanks a lot.

 

Jennifer M. Anderson, Scientist

Halozyme Therapeutics, Inc.

11404 Sorrento Valley Road

San Diego, CA 92121

858-704-8333

janderson <@t> halozyme.com

 

 


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