[Histonet] Breast tissue
laurie.reilly <@t> jcu.edu.au
Tue Feb 10 17:21:43 CST 2009
The major problem with processing fatty tissues, assuming that they are
fixed properly, is that Ethanol is not a good solvent for fat and therefore
connot penetrate the tissues completely, so the tissues are inadequately
We have had some success with lipomas by adding a "degreasing" step of
xylene into the processing schedule.
The first Absolute ethanol will dehydrate the tissue to some extent. The
next Xylene step will remove most of the fat and then the second Absolute
ethanol can complete the dehydration.
A compromise situation that we use routinely is to have Absolute ethanol,
50:50 Absolute ethanol:Xylene, Xylene This is not quite as effective but it
is less disruptive to the normal schedule and handles moderately fatty
Regards from Townsville, Australia.
Mr. Laurie REILLY
School of Veterinary and Biomedical Sciences
James Cook University
Townsville Qld. 4811
Phone 07 4781 4468
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
rmweber113 <@t> comcast.net
Sent: Tuesday, 10 February 2009 11:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Breast tissue
Hello, Do anyone have a better remedy to process fatty breast tissue
other than after processing pressing it in paper towels and then putting it
back in paraffin for a couple of hours. The pathologist are saying this is
taking to long.
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