[Histonet] Breast tissue

Angela Bitting akbitting <@t> geisinger.edu
Tue Feb 10 11:09:45 CST 2009

We extended our paraffin infiltration times and that worked out well for us too.

>>> <mari.ann.mailhiot <@t> leica-microsystems.com> 2/10/2009 11:10 AM >>>
As long as you smell the clearant in the block then putting the blocks in
paraffin longer works. I never found it necessary to roll the fat in paper
towel and place the specimen back in paraffin.  If the block is not fixed
ore dehydrated properly and cleared properly putting them in paraffin
doesn't work.

It all starts at the gross station. Cutting nice thin sections that are
fixed for at least 24 hrs. Even before that breast should be bread loafed
and placed in formalin and fixed for 24 hrs. Then a section is cut and
placed in a cassette and that sits in formalin until it gets placed on the

The process of rolling fatty tissue in paper towel and gently pressed
allows the fatty specimen to go back in formalin and be reprocessed without
taking the specimen back through the xylenes and alcohols to reprocess.

Hope this helps.

Best regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist/Trainer
Leica Microsystems
Biosystems Division
Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com 

             rmweber113 <@t> comcas                                             
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                                       [Histonet] Breast tissue            
             02/10/2009 07:49                                              

Hello,    Do anyone have a better remedy to process fatty breast tissue
other than after processing pressing it in paper towels and then putting it
back in paraffin for a couple of hours.   The pathologist are saying this
is taking to long.


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