[Histonet] HPS stain from the AFIP manual

Madary, Joseph MadaryJ <@t> MedImmune.com
Tue Feb 10 14:19:07 CST 2009


We use the HPS routinely here as well.  It is in the AFIP manual, the old green one and it did use itself as a reference, so I am glad to know how it originated.  You know I have done that procedure for nearly three decades and I have noticed in past years the quality of Saffron(crocus)from any place but ROBOZ chroma from years back is not as good.  I would love to hear of a place that sells it now that is good off the shelf.  I agree with all of the boiling, parafilm and we also place it in the oven the night before we use it.  I run the HPS every other day.  I did notice minimal difference using sat pic acid or bouins.


Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory Mgr
One Medimmune Way, Lab 2438-Area 4
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

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Sent: Tuesday, February 10, 2009 1:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 63, Issue 12

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Today's Topics:

   1. need help with Elastase staining (Patricia F Lott)
   2. HPS stain (Gagnon, Eric)
   3. Re: Disinfecting Cryostat (Caroline Miller)
   4. AO 860 repairman in NC? (Caroline Bass)
   5. Re: HPS stain (Robert Richmond)
   6. special stain instrument (Santiago, Albert)
   7. thermowave users (godsgalnow <@t> aol.com)
   8. Techs from Rockford Health System (Steven Coakley)
   9. Microtomy of LEEP specimens (Joanne Clark)
  10. RE: Microtomy of LEEP specimens (Mike Pence)
  11. Re: Microtomy of LEEP specimens (Victor Tobias)
  12. RE: Microtomy of LEEP specimens (Laurie Colbert)
  13. cotton blue=acid blue=methyl blue (Edwards, R.E.)
  14. Breast tissue (rmweber113 <@t> comcast.net)
  15. NY DOH inspection question (Whitaker, Bonnie)
  16. Re: cotton blue=acid blue=methyl blue (Geoff McAuliffe)
  17. RE: NY DOH inspection question (McMahon, Loralee A)
  18. RE: Microtomy of LEEP specimens (Smith Wanda)
  19. Re: Breast tissue (Rene J Buesa)
  20. Ventana Ultra (thisisann <@t> aol.com)
  21. spin columns (Emily Sours)
  22. spin columns (Pat Flannery)
  23. Re: Breast tissue (mari.ann.mailhiot <@t> leica-microsystems.com)
  24. RE: RE: Microtomy of LEEP specimens (Tom McNemar)
  25. Re: Breast tissue (Angela Bitting)


----------------------------------------------------------------------

Message: 1
Date: Mon, 9 Feb 2009 12:09:55 -0600
From: "Patricia F Lott" <plott <@t> uab.edu>
Subject: [Histonet] need help with Elastase staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<DCFAB2F06434294D8CF45C3E28C88D9E56C3B3 <@t> UABEXMB8.ad.uab.edu>
Content-Type: text/plain;	charset="US-ASCII"

Does anyone have any experience with staining for Elastase in Neutrophils in tissue sections?  Any help would be appreciated!



------------------------------

Message: 2
Date: Mon, 9 Feb 2009 13:11:50 -0500
From: "Gagnon, Eric" <gagnone <@t> KGH.KARI.NET>
Subject: [Histonet] HPS stain
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<F93BD6329FC3AE4C8DB116B985FBC31327C3A872 <@t> KGHMAIL.KGH.ON.CA>
Content-Type: text/plain;	charset="iso-8859-1"

Hi Sharon,
 
HPS is our routine stain (instead of H&E, which 98% of labs use).  Not sure what part of the stain you are having trouble with, but most unpredictability usually comes in the phloxine/saffron balance.  
 
Some things to watch for are: 
-keeping your saffron covered, and I would suggest parafilm-ing it when not in use -keep the alcohols before the saffron rigorously free of phloxine-contaminated alcohol, thus not allowing tainted alcohol to contaminate/dilute the saffron during staining -getting all the background hematoxylin and phloxine out, which can be checked as you go along when you validate your method -be diligent about the preparation of the saffron.  In addition to Hermina's suggested method, we microwave the dry saffron, grind it, then boil the alcoholic mixture to remove as much moisture as possible
 
What do you use the stain for, i.e. differentiating muscle and collagen?  As a routine stain, I understand its use in Canada is limited to a few laboratories in the east such as the Ottawa, Kingston, Sudbury areas.  This is often because pathologists who trained here at Queen's Pathology like to take the stain with them.  Having said that, many of our residents request slides stained with H&E, to familiarize themselves with that stain before exams.
 
Good luck, hope this helps...
 
Eric Gagnon MLT
Histology Laboratory,
Kingston General Hospital
Kingston, Ontario, Canada




------------------------------

Message: 3
Date: Mon, 9 Feb 2009 10:17:19 -0800
From: Caroline Miller <cmiller <@t> gladstone.ucsf.edu>
Subject: Re: [Histonet] Disinfecting Cryostat
To: lpwenk <@t> sbcglobal.net
Cc: histonet <@t> lists.utsouthwestern.edu, 'Ingles Claire '
	<CIngles <@t> uwhealth.org>
Message-ID: <407E13F3-8499-4B03-BB56-E7DC95A9448D <@t> gladstone.ucsf.edu>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes

In the UK (where I used to run a clinical lab) we also would turn off  
the cryostat in the morning, wait for it to defrost then just before  
going home for the evening heat up some formalin in the microwave and  
place that in the chamber and leave overnight.

It certainly kills everything, but it is also kinda toxic to your  
lungs! I wouldn't suggest it but it is a 'severe cleaning' option,  
especially if you have had something nasty in there.

This was about 5 years ago, the rules may have changed, and Yes, I do  
realise just how bad that is for you and everyone around!

Caroline


Caroline Miller
Co-Manager
J David Gladstone Institutes Histology and Microscopy Core
1650 Owens St
San Francisco
CA 94158

http://www.gladstone.ucsf.edu/gladstone/site/histology/
cmiller <@t> gladstone.ucsf.edu




On Feb 7, 2009, at 1:11 PM, Lee & Peggy Wenk wrote:

> 95-100% also work, for bacteria, fungus, viruses. They will not work  
> on
> killing spores. That's where the 70% is to be used, as the water  
> (30% part)
> is needed to soften the spore wall, so that the alcohol (70% part)  
> can get
> inside.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Hospital
> Royal Oak, MI 48073
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
> Claire
> Sent: Friday, February 06, 2009 6:38 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Disinfecting Cryostat
>
> Why no 95%? We have been using it in perpituity with no bad effects.  
> The
> alcohol has evaporated from the inside surfaces by morning.
>
> Claire
>
> ________________________________
>
> From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of gayle  
> callis
> Sent: Fri 2/6/2009 11:11 AM
> To: 'Louise Hartson'; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Disinfecting Cryostat
>
>
>
> We use 70% ethanol on gauze (just damp) to wipe down inside of  
> cryostat. Do
> not use 95% or 100% alcohol, as 70% is the most effective for  
> disinfection,
> commonly used in biohoods. We pick up little trimmings with first  
> dampened
> gauze (or kim wipe) then re-wipe with a second gauze. We prefer  
> gauze for
> its flexibility.  Also, if you put a kimwipe or gauze behind knife  
> holder to
> l catch trimmings then you can fold up kimwipe, and lift "garbage"  
> out,
> intact, and go to biohazard container before wipe down.  We try to  
> avoid too
> much alcohol on knife holder parts since alcohol ruins lubricants  
> needed for
> good operation.  Check with your cryostat manufacturer to see what  
> they
> recommend.   It pays to wipe down under and over sliding glass door to
> counterattack biohazardous "cling-ons" found on your gloves, and where
> people have touched the instrument. 70% alcohol will not kill prions.
>
> Water based disinfectants freeze in cryostat. Eventually you need  
> scheduled
> disinfection with cryostat in defrosted mode with approved  
> disinfecting
> agent.
>
>
>
>
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 4
Date: Mon, 09 Feb 2009 13:47:31 -0500
From: Caroline Bass <cbass <@t> wfubmc.edu>
Subject: [Histonet] AO 860 repairman in NC?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C5B5E473.3410%cbass <@t> wfubmc.edu>
Content-Type: text/plain;	charset="ISO-8859-1"

Hello Everyone,

I have refurbished an old AO 860 sliding microtome. I¹m pretty proud of
myself, I¹ve unlocked the totally gummed mechanism and have put it together
myself. It seems to work very well. The only problem I have is tuning the
sliding block. I have no idea how to approach it, and after several failed
attempts (I can get it to slide down the length, but can¹t achieve a smooth
movement), I¹m wondering if it¹s better to find a repairman in the area that
can spend an hour showing me how to do this.

Does anyone have suggestions? Do you know of any repairmen in the area?

Thanks,

Caroline Bass


------------------------------

Message: 5
Date: Mon, 9 Feb 2009 14:24:01 -0500
From: Robert Richmond <RSRICHMOND <@t> aol.com>
Subject: [Histonet] Re: HPS stain
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<abea52a60902091124h5d42780bg96bd654237f9cec9 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

The hematoxylin-phloxin- saffron stain, which supposedly originated at
the Montreal Neurological Institute, migrated from there to
Columbia-Presbyterian Hospital in New York City, where it was used as
the general oversight stain in surgical pathology at least into the
1960's. When I saw it in use there in 1966 they didn't seem to be
having trouble with it.

On the other side of Central Park, New York Hospital (Cornell Medical
Center) had used a Light Green trichrome stain as a general oversight
stain. I think they abandoned it in favor of H & E after Chandler
Foot's retirement in 1948.

Saffron is a natural dye derived from the stigmas of the saffron
crocus, Crocus sativus. Although it was (and is) extremely expensive,
it was used both as a textile dye and as a spice (in paella, for
example). It's sometimes seen (and smelled) as a component of
expensive curry powders - my wife and I have been experimenting with
Penzey's Maharajah curry powder, which is 2% saffron. The immense hand
labor of extracting all those stigmas from the flowers makes saffron
"the most expensive spice in the world".

Saffron as a histologic dye has traditionally been specified as
"safran du Gâtinais" - from a particular region of France - though I
am not certain that such a product still actually exists. I would
think that any good quality saffron would suffice (don't substitute
safflower, 'dyer's saffron'). You'll pay at least ten dollars a gram
for saffron, at Penzeys anyway.

Some recipes specified as many as seven changes of hot alcohol to
extract the dye, and some people used a reflux condenser for the
extraction. The alcohol extract has a strong medicinal smell which
some people find quite unpleasant.

(I have no connection with Penzeys.com, except that my wife and I have
gotten addicted to their shipments of very high quality spices.)

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

Message: 6
Date: Mon, 9 Feb 2009 14:57:24 -0500
From: "Santiago, Albert" <Albert.Santiago <@t> uphs.upenn.edu>
Subject: [Histonet] special stain instrument
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<BB0F4D2041EED9458AD95A538DBC4CAE02FA73D2 <@t> uphsmbx6.UPHS.PENNHEALTH.PRV>
	
Content-Type: text/plain;	charset="us-ascii"

Hello again fellow histonetters, I have an old artisan special stain/ihc
stain stainer that I'm looking to replace with a newer model, but before
I commit to it I was wondering if anyone else uses any other special
stain instrument that I can look into and compare.... Thanks for your
help....

 

Albert Santiago, HT (ASCP)

Laboratory Supervisor

Dermatopathology

Phone 215-662-6539

Fax     215-662-6150

 

 

 



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------------------------------

Message: 7
Date: Mon, 09 Feb 2009 15:03:47 -0500
From: godsgalnow <@t> aol.com
Subject: [Histonet] thermowave users
To: histonet <@t> pathology.swmed.edu
Message-ID: <8CB5908A749D8F9-F58-E84 <@t> webmail-da13.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

Can any ThermoWave users that microwave prostates please email me offline, if you are willing to share SOPs....


Thanks,

Roxanne


------------------------------

Message: 8
Date: Mon, 9 Feb 2009 12:04:49 -0800 (PST)
From: Steven Coakley <sjchtascp <@t> yahoo.com>
Subject: [Histonet] Techs from Rockford Health System
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <219757.8126.qm <@t> web38205.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii

Wondering if there are any tech here from Rockford Health System, Rockford, Ill.


      

------------------------------

Message: 9
Date: Mon, 9 Feb 2009 13:39:11 -0700
From: "Joanne Clark" <jclark <@t> pcnm.com>
Subject: [Histonet] Microtomy of LEEP specimens
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <0CDA5E1E01301F4880A8A7A8BCBDA39CF66519 <@t> mail.pcnm.com>
Content-Type: text/plain;	charset="us-ascii"

Hi,

 

How many of you have a protocol to cut multiple deepers/levels on LEEP
specimens and if so, how many do you cut?

 

Thanks

Joanne Clark, HT, MLT

Pathology Consultants of New Mexico

Roswell, NM



------------------------------

Message: 10
Date: Mon, 9 Feb 2009 14:59:33 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Microtomy of LEEP specimens
To: "Joanne Clark" <jclark <@t> pcnm.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<661949901A768E4F9CC16D8AF8F2838C017A3A83 <@t> IS-E2K3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

We cut 3 levels on each block.
Mike

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
Clark
Sent: Monday, February 09, 2009 2:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microtomy of LEEP specimens


Hi,

 

How many of you have a protocol to cut multiple deepers/levels on LEEP
specimens and if so, how many do you cut?

 

Thanks

Joanne Clark, HT, MLT

Pathology Consultants of New Mexico

Roswell, NM

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 11
Date: Mon, 09 Feb 2009 14:08:14 -0800
From: Victor Tobias <victor <@t> pathology.washington.edu>
Subject: Re: [Histonet] Microtomy of LEEP specimens
To: Joanne Clark <jclark <@t> pcnm.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4990A94E.9030001 <@t> pathology.washington.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

3 levels.

Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
victor <@t> pathology.washington.edu
206-598-2792
206-598-7659 Fax
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Joanne Clark wrote:
> Hi,
>
>  
>
> How many of you have a protocol to cut multiple deepers/levels on LEEP
> specimens and if so, how many do you cut?
>
>  
>
> Thanks
>
> Joanne Clark, HT, MLT
>
> Pathology Consultants of New Mexico
>
> Roswell, NM
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>   



------------------------------

Message: 12
Date: Mon, 9 Feb 2009 14:10:46 -0800
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
Subject: RE: [Histonet] Microtomy of LEEP specimens
To: "Joanne Clark" <jclark <@t> pcnm.com>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<57BE698966D5C54EAE8612E8941D768304E9D603 <@t> EXCHANGE3.huntingtonhospital.com>
	
Content-Type: text/plain;	charset="us-ascii"

Three levels on all blocks

Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne
Clark
Sent: Monday, February 09, 2009 12:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microtomy of LEEP specimens

Hi,

 

How many of you have a protocol to cut multiple deepers/levels on LEEP
specimens and if so, how many do you cut?

 

Thanks

Joanne Clark, HT, MLT

Pathology Consultants of New Mexico

Roswell, NM

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 13
Date: Tue, 10 Feb 2009 11:45:11 +0000
From: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Subject: [Histonet] cotton blue=acid blue=methyl blue
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<7722595275A4DD4FA225B92CDBF174A1745423DC64 <@t> EXC-MBX3.cfs.le.ac.uk>
Content-Type: text/plain; charset="us-ascii"


Looking  for  a  supplier please, I am  aware  that  Sigma/Fluka supply a ready made solution, which apparently does not work.

                                Many  thanks

                                         Richard  Edwards
                                            Leicester University

                                                 U.K.





------------------------------

Message: 14
Date: Tue, 10 Feb 2009 13:49:58 +0000 (UTC)
From: rmweber113 <@t> comcast.net
Subject: [Histonet] Breast tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<1347926049.554711234273798846.JavaMail.root <@t> sz0046a.westchester.pa.mail.comcast.net>
	
Content-Type: text/plain; charset=utf-8




Hello,    Do anyone have a better remedy to process fatty breast tissue other than after processing pressing it in paper towels and then putting it back in paraffin for a couple of hours.   The pathologist are saying this is taking to long. 



Thanks, 






------------------------------

Message: 15
Date: Tue, 10 Feb 2009 09:26:02 -0500
From: "Whitaker, Bonnie" <Bonnie.Whitaker <@t> osumc.edu>
Subject: [Histonet] NY DOH inspection question
To: HistoNet <@t> pathology.swmed.edu
Message-ID: <3CE20ED86C4A114EBDF3BCE8DEFD8F60801E52 <@t> msxc06.OSUMC.EDU>
Content-Type: text/plain; charset=us-ascii

Hi All,

Will some of you that are inspected by the NYDOH please email me with how you
meet the requirement (below) for performance verification for alcohol, xylene
and formalin? 
Clinical Laboratory Standards of Practice, Part 1- General Systems; Reagents
Sustaining Standard of Practice 4 (REAG S4): Inventory Control.
The standard states that the inventory log should include the following
information: lot numbers, expiration dates, the date of receipt in the
laboratory, date of performance verification and the date the material is
placed in service, it is a requirement. 


Thanks!!

Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048



------------------------------

Message: 16
Date: Tue, 10 Feb 2009 09:55:10 -0500
From: Geoff McAuliffe <mcauliff <@t> umdnj.edu>
Subject: Re: [Histonet] cotton blue=acid blue=methyl blue
To: "Edwards, R.E." <ree3 <@t> leicester.ac.uk>
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4991954E.7020401 <@t> umdnj.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Sounds like you want Aniline Blue, CI 42780. Much easier to find by that 
name.

Geoff

Edwards, R.E. wrote:
> Looking  for  a  supplier please, I am  aware  that  Sigma/Fluka supply a ready made solution, which apparently does not work.
>
>                                 Many  thanks
>
>                                          Richard  Edwards
>                                             Leicester University
>
>                                                  U.K.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff <@t> umdnj.edu
**********************************************





------------------------------

Message: 17
Date: Tue, 10 Feb 2009 09:58:43 -0500
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] NY DOH inspection question
To: "Whitaker, Bonnie" <Bonnie.Whitaker <@t> osumc.edu>,
	<HistoNet <@t> pathology.swmed.edu>
Message-ID:
	<2CF6F6B05263EA4EBAB07781B51E5DB002C945D8 <@t> e2k3ms1.urmc-sh.rochester.edu>
	
Content-Type: text/plain;	charset="iso-8859-1"

I would be interested in how you verify the performance of the xylenes and alcohols as well.  Thank you in advance.
 
Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester 
Department of Pathology
 
(585) 275-7210
 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Whitaker, Bonnie
Sent: Tue 2/10/2009 9:26 AM
To: HistoNet <@t> pathology.swmed.edu
Subject: [Histonet] NY DOH inspection question



Hi All,

Will some of you that are inspected by the NYDOH please email me with how you
meet the requirement (below) for performance verification for alcohol, xylene
and formalin?
Clinical Laboratory Standards of Practice, Part 1- General Systems; Reagents
Sustaining Standard of Practice 4 (REAG S4): Inventory Control.
The standard states that the inventory log should include the following
information: lot numbers, expiration dates, the date of receipt in the
laboratory, date of performance verification and the date the material is
placed in service, it is a requirement.


Thanks!!

Bonnie Whitaker
Clinical Histology Manager
Ohio State University Medical Center
614.293.5048

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 18
Date: Tue, 10 Feb 2009 09:06:02 -0600
From: Smith Wanda <Wanda.Smith <@t> HCAhealthcare.com>
Subject: [Histonet] RE: Microtomy of LEEP specimens
To: Joanne Clark <jclark <@t> pcnm.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<9E2D36CE2D7CBA4A94D9B22E8328A3BACBE95606 <@t> NADCWPMSGCMS03.hca.corpad.net>
	
Content-Type: text/plain; charset="us-ascii"

We routinely cut 3 levels on 3 slides for each block. 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Monday, February 09, 2009 3:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microtomy of LEEP specimens

Hi,

 

How many of you have a protocol to cut multiple deepers/levels on LEEP specimens and if so, how many do you cut?

 

Thanks

Joanne Clark, HT, MLT

Pathology Consultants of New Mexico

Roswell, NM

_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 19
Date: Tue, 10 Feb 2009 07:21:09 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Breast tissue
To: histonet <@t> lists.utsouthwestern.edu, rmweber113 <@t> comcast.net
Message-ID: <636989.62353.qm <@t> web65701.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

The "better remedy" is to cut the slices thin and fix/process correctly, specially using mineral oil.
René J.

--- On Tue, 2/10/09, rmweber113 <@t> comcast.net <rmweber113 <@t> comcast.net> wrote:

From: rmweber113 <@t> comcast.net <rmweber113 <@t> comcast.net>
Subject: [Histonet] Breast tissue
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, February 10, 2009, 8:49 AM


Hello,    Do anyone have a better remedy to process fatty breast tissue
other than after processing pressing it in paper towels and then putting it back
in paraffin for a couple of hours.   The pathologist are saying this is taking
to long. 



Thanks, 




_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



      

------------------------------

Message: 20
Date: Tue, 10 Feb 2009 10:34:13 -0500
From: thisisann <@t> aol.com
Subject: [Histonet] Ventana Ultra
To: histonet <@t> lists.utsouthwestern.edu
Cc: ali.malik <@t> ventana.roche.com
Message-ID: <8CB59AC28EAADEE-708-DC <@t> mblk-d24.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

I am considering purchasing a Ventana Benchmark Ultra IHC.? Does anyone have any feedback concerning this piece of equipment.? Any feedback would be appreciated.
Thanks,
Ann


------------------------------

Message: 21
Date: Tue, 10 Feb 2009 10:36:56 -0500
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: [Histonet] spin columns
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<b39794b0902100736k767f8bb6v827cff813efdddfc <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8

Hello

Anyone know where to purchase QIAquick spin columns? If you get them from
Qiagen, they aren't sold separately, you have to buy them in a complete
kit.  I have a feeling I'm stuck doing so.

Emily
-- 
It's like hearing Billy Joel play "Piano Man"-- joyless for all involved,
but demanded by a higher power.
--Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax


------------------------------

Message: 22
Date: Tue, 10 Feb 2009 10:58:22 -0500
From: Pat Flannery <pjfnefro <@t> duke.edu>
Subject: [Histonet] spin columns
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <64D46715-499C-48B9-8CFA-EDBEBF95B810 <@t> duke.edu>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes

Emily-

I just got a mailing from Genesee Scientific (800-789-5550, Ext. 2)  
for their UniPrep columns.  They're selling them specially for those  
people who always run out of columns before they run out of reagents.   
I think there are a couple of types, so you might want to contact them  
to see which one you'd need.

Hope this helps.

-Pat Flannery
  Duke Med Center


> Hello
>
> Anyone know where to purchase QIAquick spin columns? If you get them  
> from
> Qiagen, they aren't sold separately, you have to buy them in a  
> complete
> kit.  I have a feeling I'm stuck doing so.
>
> Emily
> -- 
> It's like hearing Billy Joel play "Piano Man"-- joyless for all  
> involved,
> but demanded by a higher power.
> --Kevin Murphy, Indiana Jones and the Crystal Skull rifftrax
> _______________________________________________



------------------------------

Message: 23
Date: Tue, 10 Feb 2009 10:10:53 -0600
From: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: Re: [Histonet] Breast tissue
To: rmweber113 <@t> comcast.net
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<OFFD3C1DAD.63E32CCA-ON86257559.0057B91E-86257559.0059237B <@t> leica-microsystems.com>
	
Content-Type: text/plain; charset=ISO-8859-1

As long as you smell the clearant in the block then putting the blocks in
paraffin longer works. I never found it necessary to roll the fat in paper
towel and place the specimen back in paraffin.  If the block is not fixed
ore dehydrated properly and cleared properly putting them in paraffin
doesn't work.

It all starts at the gross station. Cutting nice thin sections that are
fixed for at least 24 hrs. Even before that breast should be bread loafed
and placed in formalin and fixed for 24 hrs. Then a section is cut and
placed in a cassette and that sits in formalin until it gets placed on the
processor.

The process of rolling fatty tissue in paper towel and gently pressed
allows the fatty specimen to go back in formalin and be reprocessed without
taking the specimen back through the xylenes and alcohols to reprocess.

Hope this helps.

Best regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist/Trainer
Leica Microsystems
Biosystems Division
Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com


                                                                           
             rmweber113 <@t> comcas                                             
             t.net                                                         
             Sent by:                                                   To 
             histonet-bounces@         histonet <@t> lists.utsouthwestern.edu   
             lists.utsouthwest                                          cc 
             ern.edu                                                       
                                                                   Subject 
                                       [Histonet] Breast tissue            
             02/10/2009 07:49                                              
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           






Hello,    Do anyone have a better remedy to process fatty breast tissue
other than after processing pressing it in paper towels and then putting it
back in paraffin for a couple of hours.   The pathologist are saying this
is taking to long.



Thanks,




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 24
Date: Tue, 10 Feb 2009 11:36:31 -0500
From: "Tom McNemar" <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] RE: Microtomy of LEEP specimens
To: "Smith Wanda" <Wanda.Smith <@t> HCAhealthcare.com>,	"Joanne Clark"
	<jclark <@t> pcnm.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<51D5D78FBEDAEA4FBCCD9A9D44211DC505B8E0A2 <@t> lmhsmail.lmhealth.org>
Content-Type: text/plain;	charset="iso-8859-1"

Same here.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Smith
Wanda
Sent: Tuesday, February 10, 2009 10:06 AM
To: Joanne Clark; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Microtomy of LEEP specimens


We routinely cut 3 levels on 3 slides for each block. 


WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Monday, February 09, 2009 3:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microtomy of LEEP specimens

Hi,

 

How many of you have a protocol to cut multiple deepers/levels on LEEP specimens and if so, how many do you cut?

 

Thanks

Joanne Clark, HT, MLT

Pathology Consultants of New Mexico

Roswell, NM

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 25
Date: Tue, 10 Feb 2009 12:09:45 -0500
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
Subject: Re: [Histonet] Breast tissue
To: <rmweber113 <@t> comcast.net>,
	<mari.ann.mailhiot <@t> leica-microsystems.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49916E89.2B7F.00C9.0 <@t> geisinger.edu>
Content-Type: text/plain; charset=US-ASCII

We extended our paraffin infiltration times and that worked out well for us too.

>>> <mari.ann.mailhiot <@t> leica-microsystems.com> 2/10/2009 11:10 AM >>>
As long as you smell the clearant in the block then putting the blocks in
paraffin longer works. I never found it necessary to roll the fat in paper
towel and place the specimen back in paraffin.  If the block is not fixed
ore dehydrated properly and cleared properly putting them in paraffin
doesn't work.

It all starts at the gross station. Cutting nice thin sections that are
fixed for at least 24 hrs. Even before that breast should be bread loafed
and placed in formalin and fixed for 24 hrs. Then a section is cut and
placed in a cassette and that sits in formalin until it gets placed on the
processor.

The process of rolling fatty tissue in paper towel and gently pressed
allows the fatty specimen to go back in formalin and be reprocessed without
taking the specimen back through the xylenes and alcohols to reprocess.

Hope this helps.

Best regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist/Trainer
Leica Microsystems
Biosystems Division
Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com 
www.leica-microsystems.com 


                                                                           
             rmweber113 <@t> comcas                                             
             t.net                                                         
             Sent by:                                                   To 
             histonet-bounces@         histonet <@t> lists.utsouthwestern.edu   
             lists.utsouthwest                                          cc 
             ern.edu                                                       
                                                                   Subject 
                                       [Histonet] Breast tissue            
             02/10/2009 07:49                                              
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           






Hello,    Do anyone have a better remedy to process fatty breast tissue
other than after processing pressing it in paper towels and then putting it
back in paraffin for a couple of hours.   The pathologist are saying this
is taking to long.



Thanks,




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet 


______________________________________________________________________
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------------------------------

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End of Histonet Digest, Vol 63, Issue 12
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