[Histonet] query regarding IHC slide

shazana hilda hilda_1075 <@t> yahoo.com
Tue Feb 3 21:23:16 CST 2009

Dear Loralee,
Unfortunately I'm using DAB and yet still faded. This is the first time I'm facing this problem.

Thank You.

Shazana Hilda ShamsuddinUniversiti Sains Malaysia
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com

From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
To: shazana hilda <hilda_1075 <@t> yahoo.com>
Sent: Tuesday, February 3, 2009 11:47:06 PM
Subject: RE: [Histonet] query regarding IHC slide

[Histonet] query regarding IHC slide 
Are you using DAB or AEC.  AEC fades and DAB does not.  
Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester 
Department of Pathology
(585) 275-7210

 From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of shazana hilda
Sent: Mon 2/2/2009 9:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] query regarding IHC slide

Dear All,
Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it?

Any response are greatly appreciated.


Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com

Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu


More information about the Histonet mailing list