[Histonet] Muscle Gomori Trichrome

Tony Henwood AnthonyH <@t> chw.edu.au
Tue Feb 3 15:04:16 CST 2009

Possibly you have allowed the slides to air dry too long before
After cutting and allowing to dry for 10 minutes, store them at -70oC
prior to staining might solve the problem


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John
Sent: Wednesday, 4 February 2009 6:27 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Muscle Gomori Trichrome

Hi Histonians,

I have experienced some difficulties in doing snapped freeze muscle
biopsies that need to be stained with the Modified Gomori's Trichrome by
Engel, W.K., and Cunningham 1963. When I stained the frozen tissue that
has not been post or pre fixed, I am getting red areas within this
bundles that also have green in it. I know it should be solid green with
reddish nuclei and red intermyofibrillar muscle. I truly believe this is
an artifact. I checked the histonet archives and there has been much
talk about the stain but not how to correct this artifact Any help will
be appreciated.

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