[Histonet] Muscle Gomori Trichrome

[Tony Henwood]

Possibly you have allowed the slides to air dry too long before
staining.
After cutting and allowing to dry for 10 minutes, store them at -70oC
prior to staining might solve the problem

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John
Shelley
Sent: Wednesday, 4 February 2009 6:27 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Muscle Gomori Trichrome


Hi Histonians,

I have experienced some difficulties in doing snapped freeze muscle
biopsies that need to be stained with the Modified Gomori's Trichrome by
Engel, W.K., and Cunningham 1963. When I stained the frozen tissue that
has not been post or pre fixed, I am getting red areas within this
bundles that also have green in it. I know it should be solid green with
reddish nuclei and red intermyofibrillar muscle. I truly believe this is
an artifact. I checked the histonet archives and there has been much
talk about the stain but not how to correct this artifact Any help will
be appreciated.

John
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

*********************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.
**********************************************************************