[Histonet] RE: query regarding IHC slide (shazana hilda)
Lecorchick, William
wlecorch <@t> rwjuhh.edu
Tue Feb 3 12:29:55 CST 2009
What mounting media are you using? Are the slides left to dry near a UV light from a hood or cryostat?
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, February 03, 2009 12:52 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 63, Issue 4
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Today's Topics:
1. Re: IHC and ISH (Aprill Watanabe)
2. RE: NY State Inspection Checklist (Norm Burnham)
3. query regarding IHC slide (shazana hilda)
4. Re: perplexed (Piero Nelva)
5. RE: Bouin's rinsing protocol (Swain, Frances L)
6. RE: query regarding IHC slide (Smith, Allen)
7. RE: perplexed (Terri Braud)
8. Re: query regarding IHC slide (Jan Shivers)
9. RE: Who can gross?? (Ingles Claire )
10. RE: cryostat adapter (YourBiomed.Com )
11. RE: IHC and ISH (YourBiomed.Com )
12. cryostat adapter. (mari.ann.mailhiot <@t> leica-microsystems.com)
13. Re: IHC and ISH (Tora Bardal)
14. RE: Histo gel Issues (Jo Dee Fish)
15. Hoescht and Giemsa (Xenophanes _)
16. DAPI with DAB (Reza Farivar-Mohseni, Dr)
17. Self-Charging Microscope Slides (Kelvin Poon)
----------------------------------------------------------------------
Message: 1
Date: Mon, 02 Feb 2009 16:19:23 -0700
From: Aprill Watanabe <awatanabe <@t> tgen.org>
Subject: [Histonet] Re: IHC and ISH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C5ACCD8B.968B%awatanabe <@t> tgen.org>
Content-Type: text/plain; charset="US-ASCII"
Bond from Leica. It's a great machine and very user friendly. The training
was great and effective. The technical staff helps in so many ways. I've
had my machine for 3 years and would get another one if we increased our
volume.
On 2/2/09 4:16 PM, "histonet-request <@t> lists.utsouthwestern.edu"
<histonet-request <@t> lists.utsouthwestern.edu> wrote:
Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
main: 602-343-8822
Fax: 602-343-8840
awatanabe <@t> tgen.org
www.tgen.org
------------------------------
Message: 2
Date: Mon, 2 Feb 2009 17:21:50 -0600
From: "Norm Burnham" <Norm.Burnham <@t> propath.com>
Subject: RE: [Histonet] NY State Inspection Checklist
To: <thisisann <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<82C7248978CB50469FD6BA68EBBEFE67F4C8AD <@t> exchange.propathlab.com>
Content-Type: text/plain; charset="us-ascii"
http://www.wadsworth.org/labcert/clep/standards.htm
Norm Burnham
ProPath
www.ProPath.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thisisann <@t> aol.com
Sent: Monday, February 02, 2009 5:12 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] NY State Inspection Checklist
I was just informed that NY now has an inspection checklist.? Can someone let
me know where I can find it.
Thanks,
Ann
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Message: 3
Date: Mon, 2 Feb 2009 18:10:33 -0800 (PST)
From: shazana hilda <hilda_1075 <@t> yahoo.com>
Subject: [Histonet] query regarding IHC slide
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <653484.44401.qm <@t> web38805.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Dear All,
Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it?
Any response are greatly appreciated.
Regards.
Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com
------------------------------
Message: 4
Date: Tue, 3 Feb 2009 19:35:20 +1100
From: "Piero Nelva" <pieronelva01 <@t> bigpond.com>
Subject: Re: [Histonet] perplexed
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <EF2DAABFCF504D089535E9F4241961A2 <@t> pentium4>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hi Angela
There was a problem with the heating pads for a certain batch of Ventana
XT's. We had uneven heating for a week or so until the rep replaced the
entire pad. You can check if your machine is one that was made in the
offending batch. No problems since.
Regards
Piero Nelva
Anatomical Pathology
Monash Medical Centre
Victoria
Australia
----- Original Message -----
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, February 03, 2009 7:25 AM
Subject: [Histonet] perplexed
Here's one for the Ventana BenchmarkXT users out there:
We mount the patient tissue on the same slide with our control tissue. The
control stains beautifully, but there is absolutely no staining in the
patient tissue. This will happen to one or two out of 30 slides on a run. It
also happened on two different instruments. Over the last week this has
occurred 6 times. Different protocols, different machines.
I tend to think its a deparaffinization issue. Can one side of a pad heat
and the other end not reach temp? We haven't noticed that the slides moved
during staining or were not seated properly on the pad.
Help me figure this one out folks!!
Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone 570-214-9634
fax 570-271-5916
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Message: 5
Date: Tue, 3 Feb 2009 06:39:03 -0600
From: "Swain, Frances L" <SwainFrancesL <@t> uams.edu>
Subject: [Histonet] RE: Bouin's rinsing protocol
To: "Jennifer Anderson" <janderson <@t> halozyme.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5B6165D78AC14544974A844787B47E38017397395C <@t> MAIL5.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii
I have been handling Bouin fixed animal tissue for years. We removed the Bouins picric acid yellow by submerging our specimens in 70% Alcohol saturated with Lithium Carbonate. It usually takes overnight. I have had PI's bring me the samples after rinsing their samples in Lithium Carbonate solution overnight, this even worked better, after rinsing with the Lithium Carbonate saturated Aqueous Solution they placed them in 70% and brought them to me for processing. Gail 's processing is right on. Have you seen the Animal Tissue Book that Gail and Diane Sterchi published? It is very helpful. I believe you can obtain it through NSH
Frances L. Swain HT(ASCP) A. A. S.
Special Procedures Technician
Department of Orthopaedic Surgery
Center for Orthopaedic Research
Barton Research Building 2R28
4301 West Markham Street
Little Rock AR 72205
(501) 686-8739 PHONE
(501) 686-8987 FAX
swainfrancesl <@t> uams.edu email
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson
Sent: Monday, February 02, 2009 3:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Bouin's rinsing protocol
Hello.
I am looking for information regarding procedures for tissue handling
after Bouin's fixation, prior to processing in a standard automated
tissue processor (VIP). We are handling pig, mouse, rat, and human
samples, and will fix in Bouin's for 48-72 hrs. I've been told to rinse
until all yellow is washed out, and people generally rinse in 70%
alcohol or water. Sometimes it take days to wash all of the picric acid
out.
Thank you so much for your wealth of information!
Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11404 Sorrento Valley Road
San Diego, CA 92121
858-704-8333
janderson <@t> halozyme.com
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Message: 6
Date: Tue, 3 Feb 2009 08:51:26 -0500
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
Subject: RE: [Histonet] query regarding IHC slide
To: 'shazana hilda' <hilda_1075 <@t> yahoo.com>
Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E4132130AC2F764D8C173C5400D5304290E7846F34 <@t> exchsrv02.barrynet.barry.edu>
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A lot depends on the stain chosen. I have used Vector's Nova Red for the last 3 years. I have seen no fading of my older Nova Red slides.
Prof. Allen A. Smith
Barry University School of Podiatric Medicine
Miami Shores, Florida
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of shazana hilda
Sent: Monday, February 02, 2009 9:11 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] query regarding IHC slide
Dear All,
Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it?
Any response are greatly appreciated.
Regards.
Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Email: hilda_1075 <@t> yahoo.com
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------------------------------
Message: 7
Date: Tue, 3 Feb 2009 09:58:19 -0500
From: "Terri Braud" <tbraud <@t> holyredeemer.com>
Subject: [Histonet] RE: perplexed
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F3D3B1CE184AA34ABB007C3E0FDCC38403848CB6 <@t> hrex-svr.holyredeemer.local>
Content-Type: text/plain; charset="iso-8859-1"
10. perplexed (Angela Bitting)
histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC and ISH
Hi everyone,
I am currently in the process of evaluating IHC equipment to replace my
old Dako Autostainers. The pathologists I work for are interested in
bringing ISH into our lab. My questions are, what IHC instrument have
you found works the best for IHC and ISH, and what kind of training did
your personnel go through to become qualified to perform ISH?
Thank you!
Jean Taylor, HT(ASCP)QIHC
ICH Tech
Meriter Labs
Madison, WI
We had the same issue here with our slides, even though the control tissue was mounted on the same slide, we would get decent control staining but no patient, or vice versa. I spoke with several Ventana tech folk, and the one thing that they insisted on, was the brand of slide I was using. We never used the "control" slides with the little red box, just charged slides from another company than what Ventana was recommending.
As a tech with over 30 years of experience, I couldn't imagine that one plus charged slide could be so different from the other and that it would contribute that much difference to how tissue would stain. After all, the tissue was still stuck fast, and no one could explain the principle behind what they were saying.
But hey - I was wrong, wrong, wrong. Once we switched to Cardinal's Superfrost Plus slides instead of another company's "charged" slides, we never had the problem again.
It still doesn't make sense, and no one can explain why, but it did seem to make a difference.
I hope this helps you. Terri
Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital and Medical Center
1648 Huntingdon Pike
Meadowbrook, PA 19046
(215) 938-3676 phone
(215) 938-3689 fax
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Message: 8
Date: Tue, 3 Feb 2009 09:12:09 -0600
From: "Jan Shivers" <shive003 <@t> umn.edu>
Subject: Re: [Histonet] query regarding IHC slide
To: "shazana hilda" <hilda_1075 <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <69FA5AAD53D2495CAD2FF3010A6CEACA <@t> auxs.umn.edu>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Please tell us what chromogen stains and counterstains you are using, and
the type of mounting medium for the coverslip. This will help us try to
answer your question.
Jan Shivers
UMN VDL
----- Original Message -----
From: "shazana hilda" <hilda_1075 <@t> yahoo.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, February 02, 2009 8:10 PM
Subject: [Histonet] query regarding IHC slide
> Dear All,
> Is anyone has experience with faded IHC-slide stained? I've done my IHC
> stained on few markers and after 3-4 months I viewed the slide and it
> seems that the stain became faded.Does anyone has opinion regarding this
> matter and how to troubleshoot it?
>
> Any response are greatly appreciated.
>
>
> Regards.
>
> Shazana Hilda ShamsuddinUniversiti Sains Malaysia,
> Off. no: +609- 766 4440
> Fax no: +609- 765 3370
> Email: hilda_1075 <@t> yahoo.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 9
Date: Tue, 3 Feb 2009 09:39:29 -0600
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Who can gross??
To: "Judith L. Williams" <juditw <@t> u.washington.edu>, "Charles.Embrey"
<Charles.Embrey <@t> carle.com>
Cc: histonet <histonet <@t> pathology.swmed.edu>
Message-ID:
<F2F030053F9B7345831BED293A6D57E109A68F <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
That's why we always keep at least one tall person on staff to reach the top shelves, etc. :)
Claire
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Judith L. Williams
Sent: Fri 1/30/2009 11:59 AM
To: Charles.Embrey
Cc: histonet
Subject: RE: [Histonet] Who can gross??
Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/
hehehehe
Judy
On Fri, 30 Jan 2009, Charles.Embrey wrote:
> As short histo techs what kind of aids are you using, stepladders or
> stools? Sorry, I couldn't resist. There is a ton of information on the
> histonet archive about this. A search should yield all the info you
> seek and more.
>
>
------------------------------
Message: 10
Date: Tue, 3 Feb 2009 7:51:30 -0800
From: "YourBiomed.Com " <yourbiomed <@t> cox.net>
Subject: RE: [Histonet] cryostat adapter
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20090203105130.EH2TU.1015850.imail <@t> fed1rmwml37>
Content-Type: text/plain; charset=utf-8
We have the adapter here at IMEB, Inc.
Please go to www.imebinc.com for
our contact info and ask for Brad.
Thank you
------------------------------
Message: 11
Date: Tue, 3 Feb 2009 7:52:59 -0800
From: "YourBiomed.Com " <yourbiomed <@t> cox.net>
Subject: RE: [Histonet] IHC and ISH
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <20090203105259.J7M4V.1015899.imail <@t> fed1rmwml37>
Content-Type: text/plain; charset=utf-8
Jean,
I work at IMEB, Inc in San Marcos, CA.
I'm very familiar with the instruments listed in the responses to your question.
What it comes down to is cost, closed system or open system (use your reagents or theirs (Vendors).
The Leica BondMax has it strengths and weaknesses as does the Ventana and Intellipath from BioCare.
They all offer great training and I'm sure you'll get the end result (staining) to meet your needs.
But do you want to buy the majority of your reagents from the vendor selling the instrument; which can be costly...depending on your quantity of slides your lab processes or open source in which you can mix and match the reagents that you prefer. This can or in some cases may not be a cost saver.
Any questions, please contact me.
Brad
IMEB, Inc
www.imebinc.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
Sent: Monday, February 02, 2009 9:51 AM
To: ihcrg <@t> googlegroups.com; IHCRG <@t> yahoogroups.com; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC and ISH
Hi everyone,
I am currently in the process of evaluating IHC equipment to replace my old Dako Autostainers. The pathologists I work for are interested in bringing ISH into our lab. My questions are, what IHC instrument have you found works the best for IHC and ISH, and what kind of training did your personnel go through to become qualified to perform ISH?
Thank you!
Jean Taylor, HT(ASCP)QIHC
ICH Tech
Meriter Labs
Madison, WI
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Tue, 3 Feb 2009 10:06:45 -0600
From: mari.ann.mailhiot <@t> leica-microsystems.com
Subject: [Histonet] cryostat adapter.
To: lchen <@t> mednet.ucla.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF486FAE44.7512D3E8-ON86257552.005448A4-86257552.005899A2 <@t> leica-microsystems.com>
Content-Type: text/plain; charset=US-ASCII
Leslie
I contacted our collegues in Germany and was given the below nformation on
the inner demensions of the Miles adapter for the CM1850 cryostat by Leica
for use with embedding rings.
The inner dimensions of the adapter are 2,3 cm x 2,3 cm.
If you have other questions please feel free contact.
Kind Regards
Mari Ann Mailhiot BA HT ASCP
Application Specialist/Trainer
Leica Microsystems
Biosystems Division
Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com
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Message: 13
Date: Tue, 03 Feb 2009 17:40:46 +0100
From: Tora Bardal <tora.bardal <@t> bio.ntnu.no>
Subject: Re: [Histonet] IHC and ISH
To: "YourBiomed.Com" <yourbiomed <@t> cox.net>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4988738E.5030303 <@t> bio.ntnu.no>
Content-Type: text/plain; charset=UTF-8; format=flowed
Does anyone have experience with InsituPro?
http://www.intavis.com/en/In_Situ_Detection/index.php
Tora
YourBiomed.Com wrote:
> Jean,
>
> I work at IMEB, Inc in San Marcos, CA.
> I'm very familiar with the instruments listed in the responses to your question.
> What it comes down to is cost, closed system or open system (use your reagents or theirs (Vendors).
> The Leica BondMax has it strengths and weaknesses as does the Ventana and Intellipath from BioCare.
> They all offer great training and I'm sure you'll get the end result (staining) to meet your needs.
> But do you want to buy the majority of your reagents from the vendor selling the instrument; which can be costly...depending on your quantity of slides your lab processes or open source in which you can mix and match the reagents that you prefer. This can or in some cases may not be a cost saver.
>
> Any questions, please contact me.
>
> Brad
> IMEB, Inc
> www.imebinc.com
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Taylor, Jean
> Sent: Monday, February 02, 2009 9:51 AM
> To: ihcrg <@t> googlegroups.com; IHCRG <@t> yahoogroups.com; histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] IHC and ISH
>
> Hi everyone,
>
> I am currently in the process of evaluating IHC equipment to replace my old Dako Autostainers. The pathologists I work for are interested in bringing ISH into our lab. My questions are, what IHC instrument have you found works the best for IHC and ISH, and what kind of training did your personnel go through to become qualified to perform ISH?
>
> Thank you!
>
> Jean Taylor, HT(ASCP)QIHC
> ICH Tech
> Meriter Labs
> Madison, WI
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 14
Date: Tue, 3 Feb 2009 08:49:41 -0800
From: "Jo Dee Fish" <jfish <@t> gladstone.ucsf.edu>
Subject: RE: [Histonet] Histo gel Issues
To: "'pam plumlee'" <paw555 <@t> yahoo.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1DF34928912D42A39B266EEB5A28C32F <@t> JFISH>
Content-Type: text/plain; charset="us-ascii"
Dear Pam,
I've had the same problem. I called Richard-Allen just after they were
"absorbed" by Thermo Fisher and got no answers. They had never heard of
such a problem and didn't know how to solve it. I have heard from another
user that had the same exact problem. I lost precious samples, E6.5 mouse
embryos, because of this "drying out and hardening" of the histogel. I had
to stop using it all together.
If anyone has any suggestions, please let us know!
Take care Pam and all histonetters,
Jo Dee
~~Jo Dee Fish~~
Senior Research Technologist
The J. David Gladstone Institutes
Co-manager Histology and Microscopy Core
Telephone: (415) 734-2567
Fax: (415) 355-0824
E-mail: jfish <@t> gladstone.ucsf.edu
Mailing address:
The J. David Gladstone Institutes
1650 Owens Street
San Francisco, CA 94158
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of pam plumlee
Sent: Monday, February 02, 2009 1:06 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Histo gel Issues
Dear Group: I'm having problems with the processing of small tissues in
histogel. I follow the suggested embedding directions on the package and
then process the gel blocks. The results are very inconsistent-ideally,
I'll get nice soft gel blocks-but, usually in a batch of 10 I get 2 good
blocks and 8 dried up, flat hard squares. They are all handled and
processed the same. Anyone experience this before? Thanks for any input.
Pam Plumlee H.T.
Pfizer La Jolla
pam.plumlee <@t> pfizer.com
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Message: 15
Date: Tue, 3 Feb 2009 16:52:23 +0000
From: Xenophanes _ <xenophanes__ <@t> hotmail.com>
Subject: [Histonet] Hoescht and Giemsa
To: <histonet <@t> lists.utsouthwestern.edu>
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Hello all. I am excited to be a new participant of the Histonet list serve. I hope that you can provide some assistance in a little problem I am having.Simply, I wish to photograph blood smear slides (methanol fixed) with both Hoescht and Giemsa. I want to find items via normal light microscopy from the Giemsa stain and visualize the Hoescht via our UV imager on the same scope. The problem I am having is that performing the Giemsa stain first doesn't work out well because the Hoescht staining solution leeches out the Giemsa and produces washed out samples with little purple staining. I am trying to do the Hoescht stain first, but I am afraid that the Giemsa staining conditions might reduce the ability to resolve the fluorescent signal from the Hoescht dye.Furthermore, Hoescht binds DNA in the minor groove, while the Giemsa binds DNA on the phosphate groups. Does any one know about possible inhibition of binding between these two compounds since they are physically clos!
e on the DNA strand itself?Thank you again for your time and assistance!-Blood Smear-O-Rama
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Message: 16
Date: Tue, 3 Feb 2009 12:06:10 -0500
From: "Reza Farivar-Mohseni, Dr" <reza.farivar <@t> mail.mcgill.ca>
Subject: [Histonet] DAPI with DAB
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<8EA9E02DB46B6E498A9D47891BA9E98D102CB6 <@t> EXMBXVS4B.campus.mcgill.ca>
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Hello,
I'm thinking of counterstaining some DAB-stained sections with DAPI. Does anyone know if DAPI will still bind with DAB stained cells and fluoresce with permount?
Thanks,
Reza
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Message: 17
Date: Tue, 03 Feb 2009 17:42:00 +0000
From: Kelvin Poon <kelvin.poon <@t> dit.ie>
Subject: [Histonet] Self-Charging Microscope Slides
To: histonet <@t> lists.utsouthwestern.edu
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Hi all,
I was wondering if anyone was familiar with the protocol used by
manufacturers such as Menzel-Glaser regarding placing a permanent
positive charge on their glass slides. In the case of M-G they are
called Superfrost PLUS or ULTRA PLUS slides. The reason I am asking is
because I wish to positively charge /quartz/ slides, rather than glass
ones. Quartz is needed for my spectroscopy experiments. I am aware that
it is relatively easy to self-coat slides if you wish to use
poly-l-lysine or silane but both will show up as background interference
in my case. I am actually unaware if and how well quartz can actually
hold a charge. Any help appreciated!
Thanks,
Kelvin
--
_______________________________________________*
*Dr. Kelvin W. C. Poon
Postdoctoral Research Fellow
*Radiation and Environmental Science Centre*
*focas** institute***
dublin institute of technology
camden row, dublin 8
Ireland
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