[Histonet] perplexed

Piero Nelva pieronelva01 <@t> bigpond.com
Tue Feb 3 02:35:20 CST 2009

Hi Angela

There was a problem with the heating pads for a certain batch of  Ventana 
XT's.  We had uneven heating for a week or so until the rep replaced the 
entire pad.  You can check if your machine is one that was made in the 
offending batch.  No problems since.


Piero Nelva
Anatomical Pathology
Monash Medical Centre

----- Original Message ----- 
From: "Angela Bitting" <akbitting <@t> geisinger.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, February 03, 2009 7:25 AM
Subject: [Histonet] perplexed

Here's one for the Ventana BenchmarkXT users out there:

We mount the patient tissue on the same slide with our control tissue.  The 
control stains beautifully, but there is absolutely no staining in the 
patient tissue. This will happen to one or two out of 30 slides on a run. It 
also happened on two different instruments. Over the last week this has 
occurred 6 times. Different protocols, different machines.
I tend to think its a deparaffinization issue. Can one side of a pad heat 
and the other end not reach temp? We haven't noticed that the slides moved 
during staining or were not seated properly on the pad.
Help me figure this one out folks!!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916

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