[Histonet] perplexed

Laurie Colbert laurie.colbert <@t> huntingtonhospital.com
Mon Feb 2 15:00:42 CST 2009


I've had similar issues with the Ventana Nexus special stainer -
specifically on the retic and trichrome.  We have always mounted the
patient tissue on the same slide as the control tissue.  About 6 months
ago, we started have inconsistent staining.  Ventana investigated
thoroughly, but we didn't come up with any answers.  So now, we have to
stain the patient tissue separately from the control tissue - which
doubles our cost since we have two slides instead of one.  Please let me
know if you figure anything out.

Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Sent: Monday, February 02, 2009 12:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] perplexed

Here's one for the Ventana BenchmarkXT users out there:

We mount the patient tissue on the same slide with our control tissue.
The control stains beautifully, but there is absolutely no staining in
the patient tissue. This will happen to one or two out of 30 slides on a
run. It also happened on two different instruments. Over the last week
this has occurred 6 times. Different protocols, different machines.
I tend to think its a deparaffinization issue. Can one side of a pad
heat and the other end not reach temp? We haven't noticed that the
slides moved during staining or were not seated properly on the pad.
Help me figure this one out folks!!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
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