[Histonet] perplexed

[Laurie Colbert]

Angela,

I've had similar issues with the Ventana Nexus special stainer -
specifically on the retic and trichrome.  We have always mounted the
patient tissue on the same slide as the control tissue.  About 6 months
ago, we started have inconsistent staining.  Ventana investigated
thoroughly, but we didn't come up with any answers.  So now, we have to
stain the patient tissue separately from the control tissue - which
doubles our cost since we have two slides instead of one.  Please let me
know if you figure anything out.

Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Angela
Bitting
Sent: Monday, February 02, 2009 12:25 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] perplexed

Here's one for the Ventana BenchmarkXT users out there:

We mount the patient tissue on the same slide with our control tissue.
The control stains beautifully, but there is absolutely no staining in
the patient tissue. This will happen to one or two out of 30 slides on a
run. It also happened on two different instruments. Over the last week
this has occurred 6 times. Different protocols, different machines.
I tend to think its a deparaffinization issue. Can one side of a pad
heat and the other end not reach temp? We haven't noticed that the
slides moved during staining or were not seated properly on the pad.
Help me figure this one out folks!!

Angela Bitting, HT(ASCP)
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!




IMPORTANT WARNING: The information in this message (and the documents
attached to it, if any) is confidential and may be legally privileged.
It is intended solely for the addressee. Access to this message by
anyone else is unauthorized. If you are not the intended recipient, any
disclosure, copying, distribution or any action taken, or omitted to be
taken, in reliance on it is prohibited and may be unlawful. If you have
received this message in error, please delete all electronic copies of
this message (and the documents attached to it, if any), destroy any
hard copies you may have created and notify me immediately by replying
to this email. Thank you.