[Histonet] cryostats
Webb, Dorothy L
Dorothy.L.Webb <@t> HealthPartners.Com
Thu Dec 31 12:07:27 CST 2009
We defrost ours quarterly or more often if needed! We wipe it out daily with absolute alcohol and do a decontamination as necessary.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, December 31, 2009 12:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 73, Issue 40
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. cap question amp.12087 (anita dudley)
2. AW: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
(Gudrun Lang)
3. Auto Reply (Baustin)
4. Sr HTL opportunity in NC (jeri <@t> opssearchgroup.com)
5. RE: Histonet Digest, Vol 73, Issue 39, Question 10. Dermlab
(Valantou Grover)
6. Re: Histonet Digest, Vol 73, Issue 39 (rgrow <@t> bmnet.com)
7. Job In Northern NY (Alyssa Peterson)
8. Path Specimen Source Files (Feher, Stephen)
9. Formalin pigment? (Adeluwoye Oluwatosin)
10. Re: 2x3 microtomy: Adapter vs Sliding Microtome
(Anne van Binsbergen)
11. RE: Probe for mouse Y chromosome for FISH (Peter Tree)
12. Re: Formalin pigment? (Rene J Buesa)
13. Re: FNA code (Mary McCoy)
----------------------------------------------------------------------
Message: 1
Date: Wed, 30 Dec 2009 12:03:36 -0600
From: anita dudley <azdudley <@t> hotmail.com>
Subject: [Histonet] cap question amp.12087
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT122-W81A2E9EBADCF490E30AB2D1790 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
just wondering if everyone is defrosting their cryostats every week? seems like overkill, we clean ours out daily and every week clean with 100 alc. what are others doing? this is a revised question. thanks a lot
anita dudley
providence hospital
mobile
_________________________________________________________________
Your E-mail and More On-the-Go. Get Windows Live Hotmail Free.
http://clk.atdmt.com/GBL/go/171222985/direct/01/
------------------------------
Message: 2
Date: Wed, 30 Dec 2009 19:18:54 +0100
From: "Gudrun Lang" <gu.lang <@t> gmx.at>
Subject: AW: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
To: "'R C'" <ruebenjcarter <@t> gmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <393235095CC64FF18D6F370D21E71EEA <@t> dielangs.at>
Content-Type: text/plain; charset="iso-8859-1"
Have you tried to float the section first in a roomtemp. waterbath (bowl with tapwater)? There you can flatten the wrinkles with a brush, then mount it on the glass slide and bring it into the warm water. While gliding from the glass you can also use the brush to pull softly on the section to stretch it.
If the infiltrated tissue is still too soft, I suggest longer infiltration time in xylen and paraffin to get rid of the fatty part of brain tissue.
Sometimes even cutting on next day of embedding (one day waiting) helps.
Do you cool the blocks before cutting? Long enough?
For big blocks we usually use one blade for trimming and a new one for getting a thin section.
Hope this helps
Gudrun
-----Urspr?ngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von R C
Gesendet: Dienstag, 29. Dezember 2009 23:16
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome.
During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38).
In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome.
Can anyone offer any first hand experience/guidance?
Thanks
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 3
Date: Wed, 30 Dec 2009 13:29:09 -0500
From: Baustin <baustin <@t> cbgbiotech.com>
Subject: [Histonet] Auto Reply
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1000237988 <@t> mail-server.cbgbiotech.com>
Content-Type: text/plain; charset="utf-8"
Please do not respond to this automatic e-mail reply.
I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies <@t> cbgbiotech.com or fax it to 614-863-1676 Attn Ordering.
If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225.
Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG.
------------------------------
Message: 4
Date: Wed, 30 Dec 2009 13:39:13 -0500
From: <jeri <@t> opssearchgroup.com>
Subject: [Histonet] Sr HTL opportunity in NC
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <87140935C4DA4270AE1356959FA8558E <@t> D3RWK391>
Content-Type: text/plain; charset="iso-8859-1"
Now that 2009 is coming to a close, some of you might have already made a new year's resolution to find a more challenging HTL opportunity. If that is true, please note this great opportunity in a wonderful city in NC.
The right candidate will take a lead role in the development and implementation of new procedures, instrumentation, computer functionality, etc. Assumes responsibility for one or more major on-going laboratory projects or functions or the equivalent - i.e. CQI, safety, instrumentation, quality control, regulatory, administrative, staff development, computer (LIS,HIS, hospital intranet, PC applications) etc. Serves as a resource in area of responsibility. Performs testing that requires specialized knowledge within a discipline and/or crosses disciplines. Trains new employees in the theoretical and operational aspects, procedures and evaluates their work. Acts as a resource for less experienced co-workers. Assists supervisor/manager in review of laboratory reports, procedures, quality control.
5 year experience in Histology Department to include immuno histochemistry. Bachelors Degree HT or HTL (ASCP) certification.
I would be happy to share more details with anyone who might be interested.
Jeri Vitello
OPS Search Group
574.633.1231
www.opssearchgroup.com
jeri <@t> opssearchgroup.com
where OPPORTUNITY and PEOPLE meet SUCCESSFULLY
------------------------------
Message: 5
Date: Wed, 30 Dec 2009 14:28:20 -0500
From: "Valantou Grover" <vgrover <@t> polysciences.com>
Subject: [Histonet] RE: Histonet Digest, Vol 73, Issue 39, Question
10. Dermlab
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <2C12F4C64F364FCEBCAFBDC0068CA1E7 <@t> USWARD13ZFB71>
Content-Type: text/plain; charset="us-ascii"
Rebecca,
There are many companies that will set up the lab from beginning to end, TBS, Inc. has this service. You can contact them at 919-384-9393.
Valantou Grover, HT/HTL(ASCP), PA, MBA
Biosciences Product Line Manager
Polysciences, Inc.
400 Valley Road
Warrington, PA 18976
Fax: 1-800-343-3291
Phone number: 1-800-523-2575 X7418
Direct:1-215-488-7418
Cell phone: 1-215-409-8327
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: None
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 73, Issue 39
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."
Today's Topics:
1. Auto Reply (Baustin)
2. Part Time MOHS Tech Needed in Portland (Eric Weber)
3. Re: Recycling Formalin, Xylene, and Alcohol (rgrow <@t> bmnet.com)
4. 2x3 microtomy: Adapter vs Sliding Microtome (R C)
5. Re: Stain to differentiate Hemoglobin from Hemosiderin?
(Robert Richmond)
6. LEICA 2055 AUTOCUT (Lucy Zong)
7. Re: Stain to differentiate Hemoglobin from Hemosiderin?
(John Kiernan)
8. Probe for mouse Y chromosome for FISH
(birnbaumm <@t> asaf.health.gov.il)
9. FNA code (Demarinis, Carolyn)
10. Derm Lab (Eric Sulkosky)
11. Re: FNA code (DKBoyd <@t> chs.net)
12. Re: Probe for mouse Y chromosome for FISH (Xipamanine Mkuze)
13. RE: LEICA 2055 AUTOCUT (Feher, Stephen)
14. Mississippi Histotechnology Homecoming (Jerry Santiago)
----------------------------------------------------------------------
Message: 1
Date: Tue, 29 Dec 2009 13:06:33 -0500
From: Baustin <baustin <@t> cbgbiotech.com>
Subject: [Histonet] Auto Reply
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1000171728 <@t> mail-server.cbgbiotech.com>
Content-Type: text/plain; charset="utf-8"
Please do not respond to this automatic e-mail reply.
I will be out of the office until Monday, January 4, 2010. If you need to place an order, please email it to supplies <@t> cbgbiotech.com or fax it to
614-863-1676 Attn Ordering.
If you need to place a credit card order, please call 1-800-941-9484 and dial ext 201 or 225.
Please note that all orders have been confirmed. If you did not receive a fax confirmation, your order was not received by CBG.
------------------------------
Message: 2
Date: Tue, 29 Dec 2009 14:53:37 -0500
From: Eric Weber <erweber <@t> maxhealth.com>
Subject: [Histonet] Part Time MOHS Tech Needed in Portland
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9D12D4EF30176D4F839EB47F3D0E843611FAAF83 <@t> exbk2.maxhealth.com>
Content-Type: text/plain; charset="us-ascii"
MOHs Tech needed at the Portland VAMC for a Part Time Basis. Please contact me if you would be interested. Details:
Dermatology MOHS Health Technician
The Portland VA Medical Center is in need of one part-time Dermatology MOHS technician for the Dermatology Clinic, Operative Care Program at the Portland VA Medical Center, 3710 SW US Veterans Hospital Road, Portland, OR 97239.
Qualifications: Knowledge of basic methods and procedures of the Dermatology clinic. Candidate shall demonstrate skill and precision in use of the tools, materials and equipment for Dermatology clinic.
Knowledge of specialized terminology used in the specialized area of the facility where the work is performed. Strong customer service skills; detail oriented; and adaptability to work in a fast paced work environment. Candidate will serve as technical expert in the operation and maintenance of specialized equipment and instruments.
Experience: Basic computer knowledge. Candidate shall demonstrate experience and skill in cutting and preparing frozen sections of tissue in a Dermatology MOHS clinic. Familiarity with medical terminology and familiarity with routine laboratory values and abbreviations are required.
Specialty Experience (a Plus but not required): Familiarity with the VA electronic medical record system (CPRS).
Length of Assignment: One year, or longer.
On-Call Hours: Not available
Overtime: Not available
Tour of Duty: Mondays for 8 hours, (7:30am to 3:30pm), Fridays for 5 hours (7:30 am to 12:30 pm).
Eric Weber
Maxim Government Services
7227 Lee DeForest Dr
Columbia, MD 21046
phone: (410) 910-4942
toll free: (866) 260-9142
fax:(410) 953-8358
erweber <@t> maxhealth.com
CONFIDENTIALITY NOTE: This e-mail message contains confidential, privileged or otherwise protected information intended solely for the addressee. Please do not read, copy, or disseminate it unless you are the intended addressee. If you have received it in error, please call us (collect) at (410) 910-1500 and ask to speak with the message sender. Also, we would appreciate your forwarding the message back to us and deleting it from your system. Thank you.
This e-mail and all other electronic (including voice) communications from the sender's firm are for informational purposes only. No such communication is intended by the sender to constitute either an electronic record or an electronic signature or to constitute any agreement by the sender to conduct a transaction by electronic means. Any such intention or agreement is hereby expressly disclaimed unless otherwise specifically indicated.
------------------------------
Message: 3
Date: Tue, 29 Dec 2009 16:04:22 -0500
From: rgrow <@t> bmnet.com
Subject: [Histonet] Re: Recycling Formalin, Xylene, and Alcohol
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFA3E10728.1D58BC58-ON8525769B.007307A4-8525769B.0073C473 <@t> bmnet.com>
Content-Type: text/plain; charset=US-ASCII
Andria,
CBG is very reliable. I have one of the large capacity units called: Solv Solv. It's set up to run xylene on one side and alcohol on the other.
Very convenient, no flushes to do. I don't recommend recycling formalin, to much fume exposure, testing, rebuffering, etc., but this unit will work for formalin too.
Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN 37804-5016
(865) 977-4744
(865) 977-5766 Fax
You wrote:
Message: 4
Date: Mon, 21 Dec 2009 14:19:36 -0500
From: "Evans, Andria B" <aevans <@t> wellspan.org>
Subject: [Histonet] Recycling Formalin, Xylene, and Alcohol
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<A3E1DFCF072D6046AABF519AE828ADB107BF6962 <@t> EXCH4.wellspan.org>
Content-Type: text/plain; charset="iso-8859-1"
I would like to know what everyone out in histoland is using to recycle there solutions. We are looking at switching out the procyclers (that are about 10 years old) to something else. Any Pros/Cons of current methods would be great. Thanks!!
Andria B Evans, HTL(ASCP)CM
Anatomic Pathology
York Hospital
1001 S. George Street
York, PA 17405
717-851-5006
------------------------------
Message: 4
Date: Tue, 29 Dec 2009 14:16:04 -0800
From: R C <ruebenjcarter <@t> gmail.com>
Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi. I'm working on a protocol for cutting monkey brain sections to be mounted on 2x3 slides. I've read about utilizing a sliding microtome but in short, have decided to use the 2x3 adapter for a standard Microm microtome.
During microtomy I've noticed many wrinkles in the sections, particularly within the folds of the cerebellum. The wrinkles worsen as the sections float in the water bath (temp=38).
In troubleshooting, a co-worker suggests inadequate fixation. I on the other hand believe that the wrinkles relate to the Type R paraffin, which contains polymers as well as the use of the adapter versus the sliding microtome.
Can anyone offer any first hand experience/guidance?
Thanks
------------------------------
Message: 5
Date: Tue, 29 Dec 2009 17:26:22 -0500
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Stain to differentiate Hemoglobin from
Hemosiderin?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<abea52a60912291426y68ea4080h94c2f627141a1335 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Jerry Ricks, Research Scientist, University of Washington,Department of Pathology asks:
>>I've seen Prussian blue as a stain for hemosiderin but that would also
stain hemoglobin.<<
The Perls prussian blue reaction occurs with hemosiderin ("stainable
iron") but not with hemoglobin, hematin, formalin pigment, malarial pigment, or melanin.
It isn't a stain, but a reaction between ferric iron and ferrocyanide ion that produces a dense blue precipitate.
The Stainsfile page doesn't really answer your questions, and I'd suggest looking it up in some of the standard textbooks.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 6
Date: Tue, 29 Dec 2009 18:05:10 -0500
From: Lucy Zong <lucy.zong <@t> gmail.com>
Subject: [Histonet] LEICA 2055 AUTOCUT
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<8daef62e0912291505l7721b8acw2c339e97a29ddb6f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I was given a Leica 2055 microtome for our lab, however it did not come with an operators manual. Does anyone have one they could e-mail to me? Thank you
------------------------------
Message: 7
Date: Tue, 29 Dec 2009 20:37:52 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] Stain to differentiate Hemoglobin from
Hemosiderin?
To: JR R <rosenfeldtek <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <fc31fb4b684f7.4b3a68a0 <@t> uwo.ca>
Content-Type: text/plain; charset=windows-1252
Please explain. The iron in haemoglobin is tightly protein-bound and not stainable by histochemical methods for the iron in haemosiderin and ferritin. Red blood cells are, for example, Prussian-blue negative.
John Kiernan
UWO. London, Canada
== == ==
----- Original Message -----
From: JR R <rosenfeldtek <@t> hotmail.com>
Date: Monday, December 28, 2009 19:54
Subject: [Histonet] Stain to differentiate Hemoglobin from Hemosiderin?
To: histonet <@t> lists.utsouthwestern.edu
>
> I've seen Prussian blue as a stain for hemosiderin but that would also
> stain hemoglobin.
>
> Any ideas?
>
> Thanks,
>
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
> _________________________________________________________________
> Hotmail: Trusted email with Microsofts powerful SPAM protection.
>
http://clk.atdmt.com/GBL/go/177141664/direct/01/____________________________
___________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Wed, 30 Dec 2009 12:17:15 +0200
From: <birnbaumm <@t> asaf.health.gov.il>
Subject: [Histonet] Probe for mouse Y chromosome for FISH
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<054452CCC076BE4DA21E46AE95E32EC3277BE9 <@t> mail2.asaf.health.gov.il>
Content-Type: text/plain; charset="windows-1255"
Dear all
We look for Fluorescence probe of mouse Y chromosome. Which company does sale it?
Mira Birnbaum
Pathology
Asaf Hrofeh Medical Center
Israel
------------------------------
Message: 9
Date: Wed, 30 Dec 2009 08:17:46 -0500
From: "Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Subject: [Histonet] FNA code
To: <HISTONET <@t> PATHOLOGY.SWMED.EDU>
Message-ID:
<F15F698E03068A4CA11D19D3E0ED2815066E29A6 <@t> shexch1.saratogacare.org>
Content-Type: text/plain; charset="us-ascii"
Which CPT code are labs using for fine needle aspirations that are processed using thinprep technique - FNA interpretation and report-88173 or thinprep non-gyn 88112? Thank you.
This e-mail communication and any attachments may contain
confidential and privileged information for the use of the
designated recipients named above.
If you are not the intended recipient, you are hereby notified
that you have received this communication in error and that
any review, disclosure, dissemination, distribution or copying
of it or its contents is prohibited. If you have received this
communication in error, please notify Saratoga Hospital
immediately by e-mail at privacy <@t> saratogacare.org and
destroy all copies of this communication and any attachments.
------------------------------
Message: 10
Date: Wed, 30 Dec 2009 09:40:09 -0500
From: Eric Sulkosky <esulkosky <@t> gmail.com>
Subject: [Histonet] Derm Lab
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<6c3840890912300640p70132627w9befa4fcca15117f <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
RAJ,
What tips are you looking for? Are you starting from scratch with an empty
room or has the room already been equipped with ventilation, plumbing and
electrical?
Eric
------------------------------
Message: 11
Date: Wed, 30 Dec 2009 09:42:03 -0500
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] FNA code
To: "Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Cc: histonet-bounces <@t> lists.utsouthwestern.edu,
HISTONET <@t> PATHOLOGY.SWMED.EDU
Message-ID:
<OF9C7D5EE1.697484AB-ON8525769C.0050BC7A-8525769C.0050BA4F <@t> chs.net>
Content-Type: text/plain; charset="US-ASCII"
88173
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
"Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/30/2009 08:18 AM
To
<HISTONET <@t> PATHOLOGY.SWMED.EDU>
cc
Subject
[Histonet] FNA code
Which CPT code are labs using for fine needle aspirations that are
processed using thinprep technique -
FNA interpretation and report-88173 or
thinprep non-gyn 88112? Thank you.
This e-mail communication and any attachments may contain
confidential and privileged information for the use of the
designated recipients named above.
If you are not the intended recipient, you are hereby notified
that you have received this communication in error and that
any review, disclosure, dissemination, distribution or copying
of it or its contents is prohibited. If you have received this
communication in error, please notify Saratoga Hospital
immediately by e-mail at privacy <@t> saratogacare.org and
destroy all copies of this communication and any attachments.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--------------------------------------------------------------------------
Disclaimer: This electronic message may contain information that is
Proprietary, Confidential, or legally privileged or protected. It
is intended only for the use of the individual(s) and entity named
in the message. If you are not an intended recipient of this
message, please notify the sender immediately and delete the
material from your computer. Do not deliver, distribute or copy
this message and do not disclose its contents or take any action in
reliance on the information it contains.
------------------------------
Message: 12
Date: Wed, 30 Dec 2009 16:37:58 +0100
From: Xipamanine Mkuze <xipamanine <@t> gmail.com>
Subject: Re: [Histonet] Probe for mouse Y chromosome for FISH
To: birnbaumm <@t> asaf.health.gov.il
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<3cfdeb590912300737g155fbd26v1e2a7b0ed98d387d <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Cambio (http://www.cambio.co.uk)
2009/12/30 <birnbaumm <@t> asaf.health.gov.il>
> Dear all
>
> We look for Fluorescence probe of mouse Y chromosome. Which company does
> sale it?
>
> Mira Birnbaum
>
> Pathology
>
> Asaf Hrofeh Medical Center
>
> Israel
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 13
Date: Wed, 30 Dec 2009 11:43:31 -0500
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: RE: [Histonet] LEICA 2055 AUTOCUT
To: "Lucy Zong" <lucy.zong <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<73A7ED895EE0C24D9267ED814911DF1912B74914 <@t> exchange.cmc-nh.org>
Content-Type: text/plain; charset="us-ascii"
I have one for the RM2255 if you think that will help.
Steve
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lucy
Zong
Sent: Tuesday, December 29, 2009 6:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] LEICA 2055 AUTOCUT
I was given a Leica 2055 microtome for our lab, however it did not come
with an operators manual. Does anyone have one they could e-mail to me?
Thank you _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 30 Dec 2009 09:08:15 -0800 (PST)
From: Jerry Santiago <jsantiago <@t> bellsouth.net>
Subject: [Histonet] Mississippi Histotechnology Homecoming
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <861067.47883.qm <@t> web180409.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=utf-8
Mississippi Histotechnology Homecoming
Calling all Histotechnologists from the State of Mississippi. The awaited
homecoming for the Mississippi Society of Histotechnology is finally here.B
The meeting information will be available in January 2010. To receive the
information, please send an e-mail with name and address to my attention to:
jsantiago <@t> bellsouth.net.
Event: Mississippi Histotechnology Homecoming Event
Dates: March 12 b
------------------------------
Message: 6
Date: Wed, 30 Dec 2009 14:51:42 -0500
From: rgrow <@t> bmnet.com
Subject: [Histonet] Re: Histonet Digest, Vol 73, Issue 39
To: histonet <@t> lists.utsouthwestern.edu
Cc: ruebenjcarter <@t> gmail.com
Message-ID:
<OFF9EFEAEE.AB203E9A-ON8525769C.006CC514-8525769C.006D1AB2 <@t> bmnet.com>
Content-Type: text/plain; charset=US-ASCII
R C,
In past years, I've section many primate brains in normal size sections
but not whole, so this may/may not help.
If it was fixation you would not be able to get a complete section in the
first place, so your fixation should not be a problem. If it was the
adaptor on your microtome you would have other sectioning problems: wobble,
thick/thin, washboard, curved sections, uneven thickness, etc.
A couple of suggestions that might help. The suggestions should work
regardless of section thickness.
1. Try initially laying your ribbon on a dish of COLD water. With fine
forceps, (chilled too) gently stretch out your section. When you are
satisfied pick your section up from the cold and transfer it to the warm
water to complete the process. Lengthens your cutting time, but works well
if you have the time.
2. Be sure to use good qualilty high profile blades, if your
stage/faceplate will accomodate them. Surgipath has good ones. For large
sections they are more stable.
3. I did use the Surgipath Infiltration Medium for the processor. And,
yes, it does make a difference. Contact your Surgipath Sales Rep and
request "Infiltration Medium" to set up in your processor. You might be
able to get enough for a trial run. Continue to use your "Type R" for
embedding.
You may need a combination of these things to get the quality you are
looking for.
Let me know if I can help further.
Happy New Year to ALL Histonetters!
Renee Grow, BA., HT (ASCP)
rgrow <@t> bmnet.com
Histology Supervisor
Blount Memorial Hospital
907 E. Lamar Alexander Pkwy.
Maryville, TN 37804-5016
(865) 977-4744
(865) 977-5766 Fax
You wrote:
Message: 4
Date: Tue, 29 Dec 2009 14:16:04 -0800
From: R C <ruebenjcarter <@t> gmail.com>
Subject: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<2a926e3f0912291416u723bd5d4ya6b93e199b4a3d0 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi. I'm working on a protocol for cutting monkey brain sections to be
mounted on 2x3 slides. I've read about utilizing a sliding microtome but in
short, have decided to use the 2x3 adapter for a standard Microm microtome.
During microtomy I've noticed many wrinkles in the sections, particularly
within the folds of the cerebellum. The wrinkles worsen as the sections
float in the water bath (temp=38).
In troubleshooting, a co-worker suggests inadequate fixation. I on the
other
hand believe that the wrinkles relate to the Type R paraffin, which
contains
polymers as well as the use of the adapter versus the sliding microtome.
Can anyone offer any first hand experience/guidance?
------------------------------
Message: 7
Date: Wed, 30 Dec 2009 15:20:35 -0500
From: Alyssa Peterson <alyssa <@t> alliedsearchpartners.com>
Subject: [Histonet] Job In Northern NY
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<bbc6db3a0912301220y3f3f8783xce31acc2401b316b <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Allied Search Partners is currently accepting resumes for qualified
histotechnician/histotechnologist applicants for a laboratory just 30 miles
north of Schenectady, NY.
Job Description:
Histotechnologists/Histotechnicians
Shifts: 11am-7pm, Monday-Friday, Full Time.
Benefits:
Great benefit package included with offer
Please submit your resume for prescreening purposes to
alyssa <@t> alliedsearchpartners.com
Sign On Bonus: Available through Allied Search Partners
*All inquiries are always kept confidential* upon resume submission one of
our recruiters will call you for an initial phone interview. No resume will
be submitted before an initial phone interview.
Be sure to visit our website www.alliedsearchpartners.com to submit your
job search request, refer a friend for $$Cash Bonus$$, and have your resume
reviewed by our career advisors.
--
Here's to a 2010 that EXCEEDS expectations!
Alyssa Peterson
Director of Recruitment
O: 888.388.7571 x 102
F: 888.388.7572
*Be sure to visit us on the web at www.alliedsearchpartners.com
------------------------------
Message: 8
Date: Wed, 30 Dec 2009 17:16:36 -0500
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: [Histonet] Path Specimen Source Files
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<73A7ED895EE0C24D9267ED814911DF1912B74922 <@t> exchange.cmc-nh.org>
Content-Type: text/plain; charset="us-ascii"
I am interested to know if any of you out there might have a fairly
comprehensive set of Pathology Specimen source files in either Word or
Excel. We are in the process of training our Registration personnel to
be able to register and accession Pathology specimens. By increasing
our source files to make it more comprehensive, we should be able to
make it an easier, and more precise, task to accession a variety of
pathology specimens into our SoftPath system.
The source files that came with the system are general in nature so that
we can add to them based on a general root system (i.e. Respiratory,
Urinary Tract, etc.).
Thanks,
Steve
Stephen A. Feher, MS, SCT (ASCP)
Pathology Supervisor
Catholic Medical Center
100 McGregor Street
Manchester, NH 03102
603-663-6707
sfeher <@t> cmc-nh.org <mailto:sfeher <@t> cmc-nh.org>
------------------------------
Message: 9
Date: Thu, 31 Dec 2009 00:56:49 +0000
From: Adeluwoye Oluwatosin <princekunlzy <@t> gmail.com>
Subject: [Histonet] Formalin pigment?
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<786f96610912301656o71e21b25vae62e9ff644de4ed <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello, please i am working effects of various concentration of
formaldehyde on liver tissue. The sections shows some degree of
formalin pigmentation form 20 to 35% and concentrated formaldehyde.
Can anyone ofer advise as to how to quantittate o judge the varying
degree of pigmentation?
Tosin
------------------------------
Message: 10
Date: Thu, 31 Dec 2009 12:52:21 +0400
From: Anne van Binsbergen <annigyg <@t> gmail.com>
Subject: Re: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
To: gu.lang <@t> gmx.at
Cc: R C <ruebenjcarter <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu
Message-ID:
<f8332fbe0912310052xeb56b0bwce76d9f6c1578a19 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
i spent many many years handling pig, mouse and human brain, cutting
sections on 2x3 slides.
the trick is to use the tap water float out before the warm water, also to
cut sections thicker than normal - cut at 10-15 and maybe even 20 microns -
trouble is at that thickness AND it being animal tissue, the sections wash
off in a flash - unless you use adhesive and cook the sections on to the
slides gently at 32C for at least overnight
i have some 20micron silver stained cerebellum on 2x3 slides here with me
right now, 20 years later they are still good as new!!
good luck
Annie
2009/12/30 Gudrun Lang <gu.lang <@t> gmx.at>
> Have you tried to float the section first in a roomtemp. waterbath (bowl
> with tapwater)? There you can flatten the wrinkles with a brush, then mount
> it on the glass slide and bring it into the warm water. While gliding from
> the glass you can also use the brush to pull softly on the section to
> stretch it.
>
> If the infiltrated tissue is still too soft, I suggest longer infiltration
> time in xylen and paraffin to get rid of the fatty part of brain tissue.
> Sometimes even cutting on next day of embedding (one day waiting) helps.
> Do you cool the blocks before cutting? Long enough?
>
> For big blocks we usually use one blade for trimming and a new one for
> getting a thin section.
>
> Hope this helps
> Gudrun
>
>
> -----Urspr?ngliche Nachricht-----
> Von: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von R C
> Gesendet: Dienstag, 29. Dezember 2009 23:16
> An: histonet <@t> lists.utsouthwestern.edu
> Betreff: [Histonet] 2x3 microtomy: Adapter vs Sliding Microtome
>
> Hi. I'm working on a protocol for cutting monkey brain sections to be
> mounted on 2x3 slides. I've read about utilizing a sliding microtome but in
> short, have decided to use the 2x3 adapter for a standard Microm microtome.
> During microtomy I've noticed many wrinkles in the sections, particularly
> within the folds of the cerebellum. The wrinkles worsen as the sections
> float in the water bath (temp=38).
>
> In troubleshooting, a co-worker suggests inadequate fixation. I on the
> other
> hand believe that the wrinkles relate to the Type R paraffin, which
> contains
> polymers as well as the use of the adapter versus the sliding microtome.
>
> Can anyone offer any first hand experience/guidance?
>
> Thanks
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Anne van Binsbergen (Hope)
Abu Dhabi
UAE
------------------------------
Message: 11
Date: Thu, 31 Dec 2009 10:03:12 +0000
From: Peter Tree <Peter.Tree <@t> abdserotec.com>
Subject: [Histonet] RE: Probe for mouse Y chromosome for FISH
To: "birnbaumm <@t> asaf.health.gov.il" <birnbaumm <@t> asaf.health.gov.il>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EBD38EE62F777E48AF47B959994F7B455E763C5BCC <@t> srv-oxf-002.morphosys.com>
Content-Type: text/plain; charset="us-ascii"
Hi Mira,
Doubtless quite a few companies out there. Try Cambio:
http://www.cambio.co.uk/catalogue-Star_FISH_copy_Mouse_Chromosome_Specific_Probes_Biotin_Labelled_br_Concentrated_Format-1-426
Conc Mouse WCP Biotin Chromosome Y
Catalogue number
1187-YMB-02
Have a great 2010
Peter
Peter Tree
Account Manager
Custom Antibody Generation
AbD Serotec - Your first choice for antibodies!
(A Division of MorphoSys)
Endeavour House, Langford Business Park,
Langford Lane, Kidlington, Oxon. OX5 1GE.
Tel: +44 (0)1865 852700
Fax: +44 (0)1865 373899
Direct: +44 (0)1865 852724
ptree <@t> ab-direct.com
www.ab-direct.com
Before you PRINT this e-mail, think about the ENVIRONMENT.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of birnbaumm <@t> asaf.health.gov.il
Sent: Wednesday, December 30, 2009 10:17 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Probe for mouse Y chromosome for FISH
Dear all
We look for Fluorescence probe of mouse Y chromosome. Which company does sale it?
Mira Birnbaum
Pathology
Asaf Hrofeh Medical Center
Israel
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
MorphoSys UK Ltd., Registered in England No: 1604642
Registered office: Greyfriars Court, Paradise Square, Oxford OX1 1BB, UK
This message may contain confidential and/or privileged information. If you are not the addressee or authorized to receive this for the addressee, you must not use, copy, disclose or take any action based on this message or any information herein. If you have received this message in error, please advise the sender immediately by reply e-mail and delete this message. Thank you for your cooperation.
Diese E-Mail kann vertrauliche und/oder rechtlich geschuetzte Informationen enthalten. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtuemlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet.
------------------------------
Message: 12
Date: Thu, 31 Dec 2009 05:58:43 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Formalin pigment?
To: histonet <@t> lists.utsouthwestern.edu, Adeluwoye Oluwatosin
<princekunlzy <@t> gmail.com>
Message-ID: <103545.48284.qm <@t> web65713.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
First you should acquire a field micrometer. One of those that have all the field of view covered by s lattice of crossed lines forming squares.
Using a low magnification objective (10:1) count in how many squares you see the pigment and calculate in what % of the total number of squares they are.
Repeat in at least 10 areas of the section selected at random, and you will have a percentage idea of how much pigment is in your section?for the time the specimen was kept in formalin at any given concentraiton.
Ren? J.
--- On Wed, 12/30/09, Adeluwoye Oluwatosin <princekunlzy <@t> gmail.com> wrote:
From: Adeluwoye Oluwatosin <princekunlzy <@t> gmail.com>
Subject: [Histonet] Formalin pigment?
To: histonet <@t> lists.utsouthwestern.edu
Date: Wednesday, December 30, 2009, 7:56 PM
Hello, please i am working effects of various concentration of
formaldehyde on liver tissue. The sections shows some degree of
formalin pigmentation form 20 to 35% and concentrated formaldehyde.
Can anyone ofer advise as to how to quantittate o judge the varying
degree of pigmentation?
Tosin
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Thu, 31 Dec 2009 10:23:07 -0500
From: "Mary McCoy" <mmccoy <@t> lakelandregional.org>
Subject: Re: [Histonet] FNA code
To: <DKBoyd <@t> chs.net>,<histonet-bounces <@t> lists.utsouthwestern.edu>,
"Carolyn Demarinis" <cdemarinis <@t> SARATOGACARE.ORG>
Cc: HISTONET <@t> PATHOLOGY.SWMED.EDU
Message-ID: <4B3C7B3E.873D.00AB.0 <@t> lakelandregional.org>
Content-Type: text/plain; charset="us-ascii"
There is no Technical component to 88173, so how do you charge for the technical preparation of the ThinPrep?
Mary McCoy HTL(ASCP)
Supervisor of Pathology Services
Lakeland Regional Health System
St. Joseph MI 49098
(269) 982-4891
mmccoy <@t> lakelandregional.org
>>> <DKBoyd <@t> chs.net> 12/30/2009 9:42 AM >>>
88173
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
"Demarinis, Carolyn" <cdemarinis <@t> SARATOGACARE.ORG>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
12/30/2009 08:18 AM
To
<HISTONET <@t> PATHOLOGY.SWMED.EDU>
cc
Subject
[Histonet] FNA code
Which CPT code are labs using for fine needle aspirations that are
processed using thinprep technique -
FNA interpretation and report-88173 or
thinprep non-gyn 88112? Thank you.
This e-mail communication and any attachments may contain
confidential and privileged information for the use of the
designated recipients named above.
If you are not the intended recipient, you are hereby notified
that you have received this communication in error and that
any review, disclosure, dissemination, distribution or copying
of it or its contents is prohibited. If you have received this
communication in error, please notify Saratoga Hospital
immediately by e-mail at privacy <@t> saratogacare.org and
destroy all copies of this communication and any attachments.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
--------------------------------------------------------------------------
Disclaimer: This electronic message may contain information that is
Proprietary, Confidential, or legally privileged or protected. It
is intended only for the use of the individual(s) and entity named
in the message. If you are not an intended recipient of this
message, please notify the sender immediately and delete the
material from your computer. Do not deliver, distribute or copy
this message and do not disclose its contents or take any action in
reliance on the information it contains.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-------------- next part --------------
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:McCoy, Mary
TEL;WORK:982-4891
ORG:;Lab
TEL;PREF;FAX:98-8258
EMAIL;WORK;PREF;NGW:MMcCOY <@t> lakelandregional.org
N:McCoy;Mary
TITLE:Coordinator Pathology
END:VCARD
BEGIN:VCARD
VERSION:2.1
X-GWTYPE:USER
FN:McCoy, Mary
TEL;WORK;PREF:982-4891
ORG:;Lab
TEL;PREF;FAX:98-8258
EMAIL;WORK;PREF;NGW:MMcCOY <@t> lakelandregional.org
N:McCoy;Mary
TITLE:Coordinator Pathology
END:VCARD
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 73, Issue 40
****************************************
This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0
More information about the Histonet
mailing list