[Histonet] Antigen retrieval and myosin heavy chain question

[Mike Jorgensen]

Dear Histonetters,

I was wondering if anyone out there has used antigen retrieval methods to
facilitate immuno-staining of formalin-fixed skeletal muscle fibers?  I am
using ALD-58 + ABC Elite Mouse kit to stain slow-tonic fibers in fresh
amphibian muscles, but need to take a look at the fiber distribution of some
rare museum specimens.  Any suggestions are welcome. Thanks!

-Mike

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Mike Jorgensen
PhD Candidate
Department of Biological Sciences
Ohio University
Athens, Ohio 45701