[Histonet] RE: leaving IHC slides in wash buffer

[Maria Katleba]

The rule is buffer no longer than 1 hour... so it will definitely compromise your IHC stain where the antibody may already be marginal.  Sorry....
Maria Katleba HT(ASCP) MS
Napa Ca

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Edwards, Richard E.
Sent: Thursday, December 17, 2009 1:27 AM
To: 'Jennifer Campbell'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: leaving IHC slides in wash buffer

Why?, and why  introduce  such an apparently unnecessary variable?.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Campbell
Sent: 16 December 2009 18:35
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] leaving IHC slides in wash buffer

Hi Everyone,

  I have yet another IHC-related question.  So after antigen retrieval,
and then allowing my slides too cool, I place them in wash buffer before
loading them on to the autostainer.  Would leaving the slides in the
wash buffer in the fridge for an extended period of time (say, 1 week)
before continuing with immunostaining affect staining quality in any
way?

Thank you,

Jen Campbell
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