[Histonet] Repairs to equipment.

Wed Dec 16 12:34:56 CST 2009

Hi,  We use Tech One Biomedical Services, Inc. as a third party PM and
repair service. They are very nice to work with and not excessive with
their fees. We have set up PM's with them for a lot of our older
equipment. Toll free number is 866-497-3033 or www.techoneweb.com . Try
them for your repairs, we have no problems with them for the four years
we have been dealing with them.  

No trees were harmed in the sending of this email. However, many
electrons were severely inconvenienced.
Theresa A Oeler, BS
Senior Research Histologist
NSABP Pathology Laboratory
Federal North Building
1307 Federal Street, Suite 303
Pittsburgh, PA 15212
412/359-8931 Of.  412/359-3239 Fx.
theresa.oeler <@t> nsabp.org

-----Original Message-----
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histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, December 16, 2009 1:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 73, Issue 24

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Today's Topics:

   1. FW: Notch-Nodal Paper (Margaryan, Naira)
   2. RE: Blade Dispenser Thingy (Bonner, Janet)
   3. RE: Blade Dispenser Thingy (Maria Katleba)
   4. Histology Jobs Outside the USA (Maria Katleba)
   5. COX2 in mice (Bell, Pat)
   6. Anti-Flag antibody (Rachel, Rivka (NIH/NEI) [E])
   7. Leica Bond (Liz Chlipala)
   8. RE: Leica Bond (Debrosse-Serra, Beatrice)
   9. Re: FW: Notch-Nodal Paper (John Kiernan)
  10. any info on Glutathione S-Transferase Pi (GST Pi)?
      (Jennifer Campbell)
  11. Equipment Repair (Yaskovich, Ruth A (NIH/NIDCR) [E])

----------------------------------------------------------------------

Message: 1
Date: Tue, 15 Dec 2009 12:00:54 -0600
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Subject: [Histonet] FW: Notch-Nodal Paper
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<C1BA93040C6B9A4A8D84878F93FEC36A038EEC0EC7 <@t> CMHEXCC01MBX.childrensmemori
al.org>

Content-Type: text/plain; charset="us-ascii"

Hi histonetters,

I am looking for some dye to be used in vivo like cell tracker or
similar might work for identifying live cells.

Any suggestions are appreciated,
Naira

------------------------------

Message: 2
Date: Tue, 15 Dec 2009 13:51:23 -0500
From: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>
Subject: RE: [Histonet] Blade Dispenser Thingy
To: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>,
	histonet <@t> lists.utsouthwestern.edu
Message-ID:

<5F31F38C96781A4FBE3196EBC22D47807F2C06 <@t> fhosxchmb006.ADVENTISTCORP.NET>

Content-Type: text/plain; charset=iso-8859-1

I keep the dispenser in a small plastic bag that seals along with the
desiccant packages they put in the original box with the blades, (and
any other desiccant packages I can find - from coverslips, inks etc.)
As long as you keep the blades sealed with the desiccant, you shouldn't
have anymore problems.  This works really well in the cryostat chamber,
too!

Janet 

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Breeden,
Sara
Sent: Tue 12/15/2009 9:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Blade Dispenser Thingy

I have Feather dispensers with blades that I have to pry out, which is
probably not a good thing.  I've tried freezing the dispenser, putting
it in an oven for a while and whacking the darned thing on the floor
(not necessarily all at the same time..).  Have we had this discussion
before and have we solved this problem?  Help?

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

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=======================================================

------------------------------

Message: 3
Date: Tue, 15 Dec 2009 11:39:48 -0800
From: Maria Katleba <Maria.Katleba <@t> stjoe.org>
Subject: [Histonet] RE: Blade Dispenser Thingy
To: "Bonner, Janet" <Janet.Bonner <@t> FLHOSP.ORG>, "Breeden, Sara"
	<sbreeden <@t> nmda.nmsu.edu>, "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<BF297E3B9FA5A14F8A14AF49FD1A561701FB774B9F <@t> SJSNT-SCMAIL03.stjoe.org>
Content-Type: text/plain; charset="us-ascii"

I use one drop of microtome oil and they slip right out.  I mean don't
go crazy with the oil either!

Maria Katleba HT(ASCP) MS
Napa CA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bonner,
Janet
Sent: Tuesday, December 15, 2009 10:51 AM
To: Breeden, Sara; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Blade Dispenser Thingy

I keep the dispenser in a small plastic bag that seals along with the
desiccant packages they put in the original box with the blades, (and
any other desiccant packages I can find - from coverslips, inks etc.)
As long as you keep the blades sealed with the desiccant, you shouldn't
have anymore problems.  This works really well in the cryostat chamber,
too!

Janet

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Breeden,
Sara
Sent: Tue 12/15/2009 9:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Blade Dispenser Thingy

I have Feather dispensers with blades that I have to pry out, which is
probably not a good thing.  I've tried freezing the dispenser, putting
it in an oven for a while and whacking the darned thing on the floor
(not necessarily all at the same time..).  Have we had this discussion
before and have we solved this problem?  Help?

Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 4700

Albuquerque, NM  87106

505-841-2576

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

=======================================================
The information contained in this message may be privileged and/or
confidential and protected from disclosure.  If the reader of this
message is not the intended recipient or an employee or agent
responsible for delivering this message to the intended recipient, you
are hereby notified that any dissemination, distribution or copying of
this communication is strictly prohibited.  If you have received this
communication in error, please notify the sender immediately by replying
to this message and deleting the material from any computer.
=======================================================
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Notice from St. Joseph Health System:
Please note that the information contained in this message may be
privileged and confidential and protected from disclosure.

------------------------------

Message: 4
Date: Tue, 15 Dec 2009 12:05:00 -0800
From: Maria Katleba <Maria.Katleba <@t> stjoe.org>
Subject: [Histonet] Histology Jobs Outside the USA
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<BF297E3B9FA5A14F8A14AF49FD1A561701FB774BAF <@t> SJSNT-SCMAIL03.stjoe.org>
Content-Type: text/plain; charset="us-ascii"

Anyone know where you can find Histology positions OUTSIDE the USA?  I
have had several colleagues ask... and I am actually interested in this
information as well.....

Any thoughts?

Maria Katleba HT(ASCP), MS
Pathology Dept. Mgr.
Queen of the Valley Medical Center
707-294-9229 cell
707-252-4411 x3689

________________________________
Notice from St. Joseph Health System:
Please note that the information contained in this message may be
privileged and confidential and protected from disclosure.

------------------------------

Message: 5
Date: Tue, 15 Dec 2009 15:00:18 -0700
From: "Bell, Pat" <Pat.Bell <@t> ucdenver.edu>
Subject: [Histonet] COX2 in mice
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<64DB27005E2FD3439E88502D7A5C91218E0894A64B <@t> CORTEZ.ucdenver.pvt>
Content-Type: text/plain; charset="us-ascii"

Does anyone know of a good antibody for COX2 that works in the mouse?

Thank you,
Pat

Pat Bell HT(ASCP)
University of Colorado, Denver
Medical Oncology; MS 8117
12801 E 17th Ave.
Aurora, Co. 80045
303-724-6077
pat.bell <@t> ucdenver.edu<mailto:pat.bell <@t> ucdenver.edu>

------------------------------

Message: 6
Date: Tue, 15 Dec 2009 17:23:00 -0500
From: "Rachel, Rivka (NIH/NEI) [E]" <rachelr <@t> nei.nih.gov>
Subject: [Histonet] Anti-Flag antibody
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C74D7674.128C6%rachelr <@t> mail.nih.gov>
Content-Type: text/plain; charset="iso-8859-1"

Does anyone know of a good rabbit anti-Flag antibody that can be used on
transfected mouse cells?

--
Rivka A. Rachel
National Eye Institute

------------------------------

Message: 7
Date: Tue, 15 Dec 2009 16:21:12 -0700
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: [Histonet] Leica Bond
To: <histonet <@t> pathology.swmed.edu>
Message-ID:
	<EE33BE5C905A3046A7FF8F58A64C8E4B100943 <@t> server.PremierLab.local>
Content-Type: text/plain;	charset="US-ASCII"

Hey everyone 

I have a question regarding the Leica Bond IHC stainer.  We are
currently evaluating it.  Is it true that you can not use a protein
block on the instrument and if you do you have to rinse afterwards.  I
know that a lot of labs do not use protein blocks anymore but we
routinely use them here, we use a serum free protein block on all of our
IHC slides.  If there is anyone out there that is willing to give me
some advice on this?  Also if anyone wants to comment on the use or lack
of use of protein block that would be greatly appreciated also. I'm
beginning to think I may be old fashioned but I'm a bit concerned about
not using protein block, OMG I hope I'm not turning into one of those
techs who has been in the field so long that they have become resistant
to change, yikes!

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

------------------------------

Message: 8
Date: Tue, 15 Dec 2009 15:29:58 -0800
From: "Debrosse-Serra, Beatrice" <Beatrice.Debrosse-Serra <@t> pfizer.com>
Subject: RE: [Histonet] Leica Bond
To: "Liz Chlipala" <liz <@t> premierlab.com>,
	<histonet <@t> pathology.swmed.edu>
Message-ID:

<A5AD5CF370EEC64A93B5155B6739DF32B66A11 <@t> lajamrexm01.amer.pfizer.com>
Content-Type: text/plain;	charset="us-ascii"

Hi Liz, 

We have Bonds and use protein blocks all the time. It isn't part of the
kit, but you can use it in one of the open containers.

Beatrice DeBrosse-Serra
Pathology Scientist 
Pfizer Global Research & Development
CB4, 2150
10646 Science Center Drive
San Diego, CA 92121
Phone# 858-622-5986
Fax# 858-678-8290

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz
Chlipala
Sent: Tuesday, December 15, 2009 3:21 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Leica Bond

Hey everyone 

I have a question regarding the Leica Bond IHC stainer.  We are
currently evaluating it.  Is it true that you can not use a protein
block on the instrument and if you do you have to rinse afterwards.  I
know that a lot of labs do not use protein blocks anymore but we
routinely use them here, we use a serum free protein block on all of our
IHC slides.  If there is anyone out there that is willing to give me
some advice on this?  Also if anyone wants to comment on the use or lack
of use of protein block that would be greatly appreciated also. I'm
beginning to think I may be old fashioned but I'm a bit concerned about
not using protein block, OMG I hope I'm not turning into one of those
techs who has been in the field so long that they have become resistant
to change, yikes!

Thanks in advance

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 9
Date: Wed, 16 Dec 2009 00:39:53 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] FW: Notch-Nodal Paper
To: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <fc52ed8f6247c.4b282c59 <@t> uwo.ca>
Content-Type: text/plain; CHARSET=US-ASCII

Dear Naira Margaryan,

Please clarify "in vivo", which means "in alive ...".  What is your
"..."?

Do you want to stain living cells in thin-layer tissue cultures,
suspended cells (blood, haemolymph etc), or cells in whole organisms
that are small enough to live under a coverslip (such as rotifers,
little nematodes and protozoans)?  For larger animals, in vivo
microscopy is possible only at the surface (skin and other accessible
surfaces) or where there are transparent media (the eye).

Beware of adverts for fluorescent stains with catchy names! If you hope
to be a career scientist you will run into the peer review process,
which is severe and does not like results based on unexplained trade
secrets.

If you explain your question on histonet, you will get plenty of  good
advice, supplemented with peer-reviewed references that you can check.  

John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "Margaryan, Naira" <NMargaryan <@t> childrensmemorial.org>
Date: Tuesday, December 15, 2009 13:02
Subject: [Histonet] FW: Notch-Nodal Paper
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>

> Hi histonetters,
> 
> I am looking for some dye to be used in vivo like cell tracker 
> or similar might work for identifying live cells.
> 
> Any suggestions are appreciated,
> Naira
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 10
Date: Wed, 16 Dec 2009 08:20:29 -0800
From: "Jennifer Campbell" <jcampbell <@t> vdxpathology.com>
Subject: [Histonet] any info on Glutathione S-Transferase Pi (GST Pi)?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:

<5658CBDB9EAE6545ABE50D2563D81BF82FA3D1 <@t> VDXSERVER01.vdxpathology.local>

Content-Type: text/plain;	charset="us-ascii"

Hi All,

  Has anyone ever used this antibody in FFPE mouse or rat tissue?  How
easy of an antibody is it to work with?  Other comments?  We may be
doing this for an upcoming project and I just wanted to know what I
could be getting myself into.  Thanks!

Jen Campbell

------------------------------

Message: 11
Date: Wed, 16 Dec 2009 12:03:29 -0500
From: "Yaskovich, Ruth A (NIH/NIDCR) [E]"
	<ryaskovich <@t> dir.nidcr.nih.gov>
Subject: [Histonet] Equipment Repair
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<EDD146C8A3C60A4B8CFBED938898272D050F291E96 <@t> NIHMLBX01.nih.gov>
Content-Type: text/plain; charset="us-ascii"

Does anyone know of a company that repairs equipment in the Maryland
area? I'm in Bethesda and my old Tissue-Tek embedding center won't hold
the temperature.
Thanks for any help,
Ruth Yaskovich
National Institutes of Health
Bethesda, Maryland

------------------------------

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