[Histonet] leaving IHC slides in wash buffer

V. Neubert histonet.nospam <@t> vneubert.com
Wed Dec 16 12:43:09 CST 2009

I guess yes.

Unless I can say exactly which ingredient will affect my staining, I 
would not leave them in any solution for such a long time!

> Hi Everyone,
>    I have yet another IHC-related question.  So after antigen retrieval,
> and then allowing my slides too cool, I place them in wash buffer before
> loading them on to the autostainer.  Would leaving the slides in the
> wash buffer in the fridge for an extended period of time (say, 1 week)
> before continuing with immunostaining affect staining quality in any
> way?
> Thank you,
> Jen Campbell
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