[Histonet] alcian blue stain

[Amos Brooks]

Hi,
    I agree with the other suggestions to do the Alcian Blue first. I would,
however take a page from the Movat Pentachrome stain and add an alkaline
alcohol step (10% ammonium hydroxide in 95% ethanol) to convert the Alcian
Blue to an insoluble stain (monastral fast blue). It may not bleach out in
subsequent steps, but this step will make sure of it.

Amos


Message: 11
Date: Mon, 14 Dec 2009 10:17:34 -0600
From: "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu>
Subject: [Histonet] alcian blue stain
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We are really getting new research procedures lately.  As with almost all
published papers the methods are a quick overview without details.  We have
been asked to do a combination stain of H&E with alcian blue.  Would you
recommend doing the alcian blue 8GX pH 2.5 first, doing it after the
hematoxylin or after the eosin?  I'm concerned about the eosin bleeding out
and the possible change in pH affecting the stains.

Margaret Perry