[Histonet] IHC macrophage marker for demineralized bone

Rene J Buesa rjbuesa <@t> yahoo.com
Mon Dec 14 15:48:26 CST 2009

Since Immuno-cal is a commercial decalcifyer it probably was developed after testing and is probably good, but I always used EDTA as a chelating agent for IHC.
René J.

--- On Mon, 12/14/09, Michele Wich <mwich <@t> 7thwavelabs.com> wrote:

From: Michele Wich <mwich <@t> 7thwavelabs.com>
Subject: [Histonet] IHC macrophage marker for demineralized bone
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, December 14, 2009, 4:30 PM

Can anyone recommend an antibody against rat macrophages which will work
in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the
best decalcifying solution for IHC procedures?

I would greatly appreciate any suggestions.

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