[Histonet] IHC macrophage marker for demineralized bone

Liz Chlipala liz <@t> premierlab.com
Mon Dec 14 15:46:42 CST 2009

F4/80 does work with FFPE formic acid decalcifed samples.


From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Michele Wich
Sent: Mon 12/14/2009 2:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC macrophage marker for demineralized bone

Can anyone recommend an antibody against rat macrophages which will work
in FFPE de-mineralized bone sections? F4/80 possibly? Is Immuno-cal the
best decalcifying solution for IHC procedures?

I would greatly appreciate any suggestions.

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