[Histonet] alcian blue stain

[Perry, Margaret]

We are really getting new research procedures lately.  As with almost all published papers the methods are a quick overview without details.  We have been asked to do a combination stain of H&E with alcian blue.  Would you recommend doing the alcian blue 8GX pH 2.5 first, doing it after the hematoxylin or after the eosin?  I'm concerned about the eosin bleeding out and the possible change in pH affecting the stains.

Margaret Perry