[Histonet] alcian blue stain

Perry, Margaret Margaret.Perry <@t> sdstate.edu
Mon Dec 14 10:17:34 CST 2009

We are really getting new research procedures lately.  As with almost all published papers the methods are a quick overview without details.  We have been asked to do a combination stain of H&E with alcian blue.  Would you recommend doing the alcian blue 8GX pH 2.5 first, doing it after the hematoxylin or after the eosin?  I'm concerned about the eosin bleeding out and the possible change in pH affecting the stains.

Margaret Perry

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