[HISTONET] fixation and storage of tissue for TEM

[Nicholas David Evans]

Dear all,

 

I would like to fix and preserve mouse skin tissue for processing for
transmission electron microscopy (TEM) at a later date. I was wondering
whether I can store tissue following fixation overnight in 2.5%
gluteraldehyde in 0.1M sodium cacodylate buffer and, if so, whether it can
be stored indefinitely in this buffer (or whether I need to treat with
OsO4 and store at 70% ethanol)?

 

Thanks and best wishes

Nick