[Histonet] MMA Sections

Jack Ratliff ratliffjack <@t> hotmail.com
Tue Dec 1 11:52:07 CST 2009


How about coating your slides with Haupt's. I have used this solution  
exclusively for all my undemineralized thin section bone work. Also, I  
only need to dry my slides overnight using a dense aluminum slide  
press (available via Dorn and Hart Microedge). Basically, the slide  
press acts to evenly flatten and "press" the sections to the Haupt's  
coated slide and also evenly heat all the slides together. Because of  
it's heat conducting properties, the press acts as a mini-oven within  
the oven to speed up the drying and section adhesion process. Please  
feel free to contact me if you have any questions regarding this or  
any other resin related topic.


Jack Ratliff
NSH Hard Tissue Committee Chair

On Dec 1, 2009, at 9:52 AM, "Karen Hughes" <hughesk <@t> upstate.edu> wrote:

> I am currently embeding 2cm sections of adult rat spine in MMA  
> including
> muscle, bone and spinal cord.  I then cut 5um sections using a  
> tungsten
> carbide D profile knife on a manual microtome and mount the sections  
> on APES
> coated slides, press them in a vise between tongue depressors, and  
> bake them
> @ 41'C for a min. of 48hrs.  My problem is that when I de-placticize  
> the
> slides in Xylene (4 changes, 2x5min, 2x10min) the spinal cord falls  
> off the
> slide, while the bone stays secure.
> I have also tried PolyLysine coated slides, SuperFrost Plus, and  
> Probe On
> Slides.  All with the same results.
> Does anyone have any experience with this?  Any and all Suggestions  
> would be
> greatly appreciated.
> Karen
> Karen S. Hughes
> Research Support Specialist
> SUNY Upstate Medical University
> Phone (315)464-8585
> hughesk <@t> upstate.edu
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