[Histonet] Special stains and microwave

Akemi Allison-Tacha akemiat3377 <@t> yahoo.com
Thu Aug 27 18:27:08 CDT 2009

Hi Rosa,

Excuse me if I am wrong, but I think Bill is referring to using a microwave for special stains, and you are referring to a Microwave Tissue Processor.  

I have used an oven set at a higher temperature (85 -110 degrees C) for staining the silver portion of the GMS, and it generally only takes between 10-15 minutes.  That is if you place the silver solution in the oven for approximately 10 minutes prior to use.  I also use the oven set at 60-62 degrees C. for the bouins portion of the Trichrome stain.  Although using a water bath at 60 degrees C. is the preferred method.  

You have more control using a oven than using a microwave.  With a microwave, results vary with the # of slides placed in the coplin jar, as well as the placement in the microwave.  There are variations in microwave intensities if you don't have a carousel.  You also may have boil over of solutions.  Food for thought.


Akemi Allison-Tacha BS, HT(ASCP)HTL
Histology Manager
APMG Laboratories
105A Cooper Court, Los Gatos, CA 95032
Contact: 800.848.2764
V/M: 408.884.2718
Fax: 408.884.2758
Cell: 408.335.9994
(W) E-Mail: aallison-tacha <@t> apmglab.com
(P) E-Mail: akemiat3377 <@t> yahoo.com

--- On Thu, 8/27/09, Rosa Fields <rfields <@t> gidocs.net> wrote:

From: Rosa Fields <rfields <@t> gidocs.net>
Subject: RE: [Histonet] Special stains and microwave
To: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>, histonet <@t> lists.utsouthwestern.edu
Date: Thursday, August 27, 2009, 2:26 PM

I can highly recommend the EBS processor microwave.  At a base price of 8,900 (last I checked) it is one of the lowest cost processors on the market.  I don't know if having the flexibility to process in this new microwave may help justify the price?  I purchased mine from Stat Lab, and the customer service from the folks at EBS was outstanding; they even had a prob and air agitator block style holder designed and built for me when I suggested that it should come with one.  I really like our model, and it offers great flexibility in our lab.  
Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE  68503
rfields <@t> gidocs.net

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Thursday, August 27, 2009 4:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Special stains and microwave

Greetings Histonetters,

We have finally come to the point where we need to fish or cut bait in
relation to purchasing a laboratory approved microwave and venting it. 

While I think it would be "cool" to have one, I'm wondering if the cost
is justified to do a handful of special stains that could be done
(though more slowly) in a laboratory oven like the one we already have.

Anyone with the time or desire to opine, please chime in.

Those who have purchased such a microwave, I'll gladly take suggestions.

Thanks in advance

William (Bill) O'Donnell, HT (ASCP) QIHC 
Lead Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

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