[Histonet] Re: DIF tissue in GLUT

Johnson, Teri TJJ <@t> stowers.org
Thu Aug 27 12:18:47 CDT 2009


Tissue that has been fixed in glutaraldehyde has very, VERY bright autofluorescence. Unless there is some way to minimize this (none that I'm aware of), your immunofluorescence will be impossible to read over the background signal.

Teri Johnson, HT(ASCP)QIHC
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64110

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