[Histonet] RE: Double HRP immunostains

C.M. van der Loos c.m.vanderloos <@t> amc.uva.nl
Thu Aug 27 07:38:31 CDT 2009

Andrea,Go for: Vector Blue (Vector Labs) or Permanent Blue (Diagnostic Biosystems) for AP activityVector Red or Permanent Red, also for AP activity. These two AP immunostaining methods can be combined in a sequential double staining method with a HIER step (10 min, 98C) in between for removing the immunoreagents. Both red or blue reaction products survive the HIER step without damage (see JOH 31:119-127). Cheers, ChrisChris van der Loos, PhD
Dept. of Pathology
Academic Medical Center M2-230
Meibergdreef 9
NL-1105 AZ Amsterdam
The Netherlands Date: Wed, 26 Aug 2009 14:05:41 -0400
From: Andrea Hooper <anh2006 <@t> med.cornell.edu>
Subject: [Histonet] Double HRP immunostains
To: Histonet <histonet <@t> lists.utsouthwestern.edu>

I do double HRP immunostains. Works well using DAB/AEC and True Blue 
... but what are your favorite chromagens to use? Would love some 
feedback for inspiration.


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