[Histonet] bubbles under coverslip

John Kiernan jkiernan <@t> uwo.ca
Wed Aug 26 12:04:45 CDT 2009

The cause of your bubble trouble is replacing xylene with a liquid whose identity is unknown and whose physical properties are obviously different. For an important application such as research or diagnosis it is not scientifically or ethically justifiable to use unknown chemicals. 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: "FU,DONGTAO" <fudo <@t> ufl.edu>
Date: Tuesday, August 25, 2009 15:13
Subject: [Histonet] bubbles under coverslip
To: histonet <@t> lists.utsouthwestern.edu

> Hi, all
>   Recently we met a problem of bubbles under coverslip 
> after we 
> changed to Xylene Substitute(from Shandon) instead of Xylene. 
> The 
> bubbles immediately appear after coverslipping. At very 
> beginning, 
> we thought it was due to mounting medium. So we changed to 
> Shandon 
> Xylene Substitue Mountant, but it did not help. Right now we 
> have 
> to transfer slides from Xylene Substitute to xylene, then 
> coverslip them. We hate the extra xylene step!
>   Does anyone know what cause the bubbles? And how to avoid 
> it? 
> Any suggestions will be appreciated.
> Ann Dongtao Fu
> Dept. of Pathology
> University of Flodrida
> Gainesville, FL 32610
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