[Histonet] FISH probe labeling

[Jurcisek, Joseph]

I have used the ULYSIS Nucleic Acid Labeling Kit from Invitrogen.  They make it with many different fluorochromes, I have used the AlexaFluor 488 and AlexaFluor 594 with good results.

Joseph A. Jurcisek
Senior Research Associate
Center for Microbial Pathogenesis
The Research Institute at Nationwide Children's Hospital
700 Children's Dr.
Columbus, OH 43205-2696
(614) 355-3521
fax (614) 722-2818
Joseph.Jurcisek <@t> nationwidechildrens.org
-----Original Message-----
From: FU,DONGTAO [mailto:fudo <@t> ufl.edu] 
Sent: Tuesday, August 25, 2009 2:58 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FISH probe labeling

Hi, All

  We are going to do FISH on mouse tissue. But we have to 
fluorescent label cDNA probe(~2kb in length) by ourselves because 
there is no commercial probe is available.

  Does anyone give me some suggestions? Like which company's 
product is suitable for labeling DNA probe? Which labeling methods 
is better? End labeling or multi?

  Thank you,

Ann Dongtao Fu
Dept. of Pathology
University of Flodrida
Gainesville, FL 32610



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