[Histonet] Caspase 3 staining in frozen tissue

[laurie <@t> conxis.com]

Hello everyone, 

Is there anyone out there doing Caspase 3 IHC on frozen tissue?  I am attempting to restandardize our protocol because the antibody we were using has stopped working.  We are using acetone fixed frozen human tonsil. 4% PFA postfixation for 10 minutes.  Using a rabbit polyclonal antibody from Abcam and Dako reagents on an autostainer.

Thanks for your ideas because mine aren't staining :-(.

Laurie