[Histonet] Caspase 3 staining in frozen tissue

laurie <@t> conxis.com laurie <@t> conxis.com
Tue Aug 25 14:00:21 CDT 2009

Hello everyone, 

Is there anyone out there doing Caspase 3 IHC on frozen tissue?  I am attempting to restandardize our protocol because the antibody we were using has stopped working.  We are using acetone fixed frozen human tonsil. 4% PFA postfixation for 10 minutes.  Using a rabbit polyclonal antibody from Abcam and Dako reagents on an autostainer.

Thanks for your ideas because mine aren't staining :-(.


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