[Histonet] Trouble making a good Cell Block

[DKBoyd <@t> chs.net]

Hi Maria,
Are you saying you are using formalin as a preservative for non-gyn's?  We 
collect all aspirations in Plasmalyte which is an electrolytic balance for 
infusion.  All other fluids are collected in a green top, sodium 
heparinized tube.  Bronch specimens are sent in saline. Urine and CSF are 
sent fresh.
When a cell block is requested we centrifuge the sediment to get a button. 
Pour off the supernate.  Then we use HistoGel to make the button.  As 
indicated, HistoGel is a gel you heat up in the microwave.  Pour off an 
equal amount into the non-gyn button let it solidify then you process the 
solid material just as any other specimen.
Hope this helps.

Debbie M. Boyd, HT(ASCP) I Chief Histologist I Southside Regional Medical 
Center I 
200 Medical Park Boulevard I Petersburg, Va.  23805 I T: 804-765-5050 I F: 
804-765-5582 I dkboyd <@t> chs.net







Maria Katleba <Maria.Katleba <@t> stjoe.org> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
08/25/2009 01:00 PM

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Subject
[Histonet] Trouble making a good Cell Block








Hi All,

Can anyone give me a good protocol for making a cell block from a non-gyn 
fluid?  (abdominal fluid, bronchial wash, pleural fluid, etc)

First of all, we have switched to formalin. Yes! Formalin!! So as you 
would expect, no button forms.

The reason? The pathologists believe the formalin is better than alcohol 
(95%) especially when you expect to run IHCs on the cell blocks.

Please send me ideas!!!!

Maria Katleba HT(ASCP) MS



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