[Histonet] glyerin/phenol storage

[Geoff McAuliffe]

Hi Jan:

We used to use glycerine+phenol  for storing slices of embalmed cadavers 
before embedding in clear plastic. I don't know the exact ratio but it 
was mostly glycerin. We did not stain but painted the nerves, blood 
vessels, etc as a teaching tool.
As for IHC, it would depend on the fixation and the antigen in question.

Geoff

Jan Shivers wrote:
> I'm posting this for a colleague....
>
> Does anyone have experience using glycerin/phenol as a storage medium, and if so, for how long are the tissues still good for Histology processing/staining?  I assume they can't be used for IHC.
>
> Thanks in advance,
>
> Jan Shivers
> University of Minnesota
> Veterinary Diagnostic Laboratory
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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