[Histonet] destaining bone

[John Kiernan]

Acid-alcohol should do the trick if you don't mind decalcifying the bone at the same time. If the basic fuchsine is for showing micro-cracks, decalcification might not be a good idea, so how about domestic bleach (sodium hypochlorite)?
 
John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: John Baker <bakerj <@t> umich.edu>
Date: Wednesday, August 19, 2009 14:09
Subject: [Histonet] destaining bone
To: Histonet <@t> lists.utsouthwestern.edu

> Hello All,  I hope someone can assist us with a protocol 
> for  
> destaining some mouse ulna.  A student stained his bones 
> for 12 hours  
> in 1% basic fuchsin in graded ethyl alcohol from 80 % , 90 % and 
> then  
> 100% as a prestain then into 100 % ethyl alcohol to destain some 
> prior  
> to pmma plastic embedding and sectioning to look at microdamage 
> in the  
> cortical bone.  The bones are over stained and if possible 
> we would  
> like to destain the bone and continue on without getting 
> more  
> specimens.  Any suggestions are welcome.  Thank you in 
> advance. John  
> Baker and Mathieu Davis
> 
> John A. Baker
> The University of Michigan
> Orthopaedic Research Laboratories
> Histology Unit
> 109 Zina Pitcher Place, 2218 BSRB
> Ann Arbor, MI 48109-2200
> 734-936-1635
> 
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