[Histonet] destaining bone

John Baker bakerj <@t> umich.edu
Wed Aug 19 13:07:27 CDT 2009

Hello All,  I hope someone can assist us with a protocol for  
destaining some mouse ulna.  A student stained his bones for 12 hours  
in 1% basic fuchsin in graded ethyl alcohol from 80 % , 90 % and then  
100% as a prestain then into 100 % ethyl alcohol to destain some prior  
to pmma plastic embedding and sectioning to look at microdamage in the  
cortical bone.  The bones are over stained and if possible we would  
like to destain the bone and continue on without getting more  
specimens.  Any suggestions are welcome.  Thank you in advance. John  
Baker and Mathieu Davis

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
109 Zina Pitcher Place, 2218 BSRB
Ann Arbor, MI 48109-2200

More information about the Histonet mailing list