[Histonet] Amyloid staining on frozen muscle bx's

Bryan Llewellyn llewllew <@t> shaw.ca
Thu Aug 20 10:21:10 CDT 2009

The method at the address below should work on cryostat sections.


Bryan Llewellyn

----- Original Message ----- 
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
To: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>; 
<histonet <@t> pathology.swmed.edu>
Sent: Wednesday, August 19, 2009 3:26 PM
Subject: RE: [Histonet] Amyloid staining on frozen muscle bx's


For successful amyloid staining with Congo red, formalin fixation might
be required.
I have not been able to find any references for this except that
formalin is used in the demonstration of amyloid in fat aspirations
(reference: Duston et al (1987) Am J Med 82:412-414)

Have you tried one of the metachromatic dyes (eg Crystal Violet)?


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Sent: Thursday, 20 August 2009 6:57 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Amyloid staining on frozen muscle bx's

What stain works for amyloid on frozen muscle bx tissue?  I did the a
Congo Red stain without much success. Thanks Sharon sallen <@t> hsc.mb.ca

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