[Histonet] Amyloid staining on frozen muscle bx's

[Bryan Llewellyn]

The method at the address below should work on cryostat sections.

http://stainsfile.info/StainsFile/stain/amyloid/siriusllew.htm

Bryan Llewellyn


----- Original Message ----- 
From: "Tony Henwood" <AnthonyH <@t> chw.edu.au>
To: "Sharon Allen" <SAllen <@t> exchange.hsc.mb.ca>; 
<histonet <@t> pathology.swmed.edu>
Sent: Wednesday, August 19, 2009 3:26 PM
Subject: RE: [Histonet] Amyloid staining on frozen muscle bx's


Sharon,

For successful amyloid staining with Congo red, formalin fixation might
be required.
I have not been able to find any references for this except that
formalin is used in the demonstration of amyloid in fat aspirations
(reference: Duston et al (1987) Am J Med 82:412-414)

Have you tried one of the metachromatic dyes (eg Crystal Violet)?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Allen
Sent: Thursday, 20 August 2009 6:57 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Amyloid staining on frozen muscle bx's


Hi,
What stain works for amyloid on frozen muscle bx tissue?  I did the a
Congo Red stain without much success. Thanks Sharon sallen <@t> hsc.mb.ca

*********************************************************************
This email and any files transmitted with it are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. 
If you are not the intended recipient, please delete it and notify the 
sender.

Views expressed in this message and any attachments are those of the 
individual sender, and are not necessarily the views of The Children's 
Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens 
Hospital at Westmead accepts no liability for any consequential damage 
resulting from email containing computer viruses.
**********************************************************************


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet