[Histonet] Amyloid staining on frozen muscle bx's

[Tony Henwood]

Sharon,

For successful amyloid staining with Congo red, formalin fixation might
be required.
I have not been able to find any references for this except that
formalin is used in the demonstration of amyloid in fat aspirations
(reference: Duston et al (1987) Am J Med 82:412-414)

Have you tried one of the metachromatic dyes (eg Crystal Violet)?

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
Allen
Sent: Thursday, 20 August 2009 6:57 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] Amyloid staining on frozen muscle bx's


Hi,
What stain works for amyloid on frozen muscle bx tissue?  I did the a
Congo Red stain without much success. Thanks Sharon sallen <@t> hsc.mb.ca

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