[Histonet] CD31(PECAM-1) by novocastra on FFPE rabbit tissue

[sotiris lakis]

Hi,

I have question about CD31(PECAM-1) by novocastra. Has anybody worked with it  on FFPE rabbit tissue? I know for instance that DAKO's works, but could find no references for the one by novocastra. Moreover I am using a Bond protocol that comes with an antimouse/antirabbit IgG 2nd Ab. I could also use some suggestions about a good blocking reagent for rabbit. Company tells me I need another detection system but that's not an option...So any protocol manipulation ideas are more than wellcome!  

Thank you in advance

Sotiris Lakis 
Department of Pathology 
G.Gennimatas Community Hospital
Thessaloniki 54635 
Hellas (Greece)     




      
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