[Histonet] Can DAB block an epitope??

Rene J Buesa rjbuesa <@t> yahoo.com
Fri Aug 14 11:55:58 CDT 2009

Theoretically, and depending on the reaction specificity, DAB should not block the epitope.
René J.

--- On Fri, 8/14/09, GT Hebert <emerald_lake77 <@t> yahoo.com> wrote:

From: GT Hebert <emerald_lake77 <@t> yahoo.com>
Subject: [Histonet] Can DAB block an epitope??
To: Histonet <@t> lists.utsouthwestern.edu
Date: Friday, August 14, 2009, 11:37 AM

Hello all,
I was hoping to run a chromogenic immuno to check if two proteins colocalize.  
Since both antibodies are made in rabbit, I was going to stain one and visualize with DAB and then heat retrieve (pressure or steam) to drop off the first antibody and restain with my second rabbit antibody using a different color to visualize.
How likely, if at all will the DAB precipitate block / mask the epitope of my second antibody if indeed they are in a similar location?
Any info would be greatly appreciated.
Thank you!
Gustave T. Hebert
Research Scientist I
Metabolic Disease Research
Wyeth Research
200 CambridgePark Drive
Cambridge, MA 02140

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