[Histonet] RE: Histonet Digest, Vol 69, Issue 9

[Pamela Gholston]

Hello Histotechs!!  Has anyone had any problems with the "cornflaking
artifact?" It looks as though the tissue has brown artifact on it.  Some of
the slides look like there is water in the tissue.  It seems like a
potential xylene purity problem.  Can I get some opinions?

Histopathy

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Subject: Histonet Digest, Vol 69, Issue 9

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Today's Topics:

   1. Good Fibrin stain with Movat's? (Amos Brooks)


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Message: 1
Date: Sun, 9 Aug 2009 07:03:07 -0400
From: Amos Brooks <amosbrooks <@t> gmail.com>
Subject: [Histonet] Good Fibrin stain with Movat's?
To: juditw <@t> u.washington.edu, histonet <@t> lists.utsouthwestern.edu
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	<582736990908090403p616a1e85ta64b1cd546bdd0c0 <@t> mail.gmail.com>
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Hi,
   Have you tried a Sirius red (in sturated picric acid) with a Weigart's
hamatoxylin for the Nucleii? I usually don't bother counterstaining the
nucleii  with this stain, but I suppose it would work. You wouuld probably
want to do the hematoxylin after the Sirius red since it is an acidic
solution and would bleach out the hematoxylin otherwise. While this isn't a
Movat's I think it might do the trick for you.

Amos

Message: 2
Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT)
From: "Judith L. Williams" <juditw <@t> u.washington.edu>
Subject: [Histonet] Good Fibrin stain with Movat's?
To: histonet <@t> lists.utsouthwestern.edu
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       <Pine.LNX.4.43.0908071021110.27227 <@t> hymn33.u.washington.edu>
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Greetings to all histonetters- I have a pathologist that wants to show
fibrin in lung. She wants also black nuclei and we tried  a Movat's to look
at fibrin. The fibrin in the lung is showing up light blue not red!
Any suggestions on how to show fibrin distinctly, with black nuclei?
thanks!
Judy


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End of Histonet Digest, Vol 69, Issue 9
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