[Histonet] Re: Specimen Release

Mon Aug 10 15:22:50 CDT 2009

Laurie,
     I can fax you a copy of a release form if you want.

Garret Miyamoto
Tripler Army Medical Center

----- Original Message -----
From: histonet-request <@t> lists.utsouthwestern.edu
Date: Saturday, August 8, 2009 7:03 am
Subject: Histonet Digest, Vol 69, Issue 8
To: histonet <@t> lists.utsouthwestern.edu


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> Today's Topics:
> 
>   1. Specimen Release (Laurie Colbert)
>   2. Good Fibrin stain with Movat's? (Judith L. Williams)
>   3. Thomas Crowell is out of the office. 
> (thomas.crowell <@t> novartis.com)   4. Re: Specimen Release (Richard 
> Cartun)   5. Peggy-cement in bone (Vicki Kalscheur)
>   6. Stain for decalcified GMA embedded bone (Yash Kolambkar)
> 
> 
> -------------------------------------------------------------------
> ---
> 
> Message: 1
> Date: Fri, 7 Aug 2009 10:17:21 -0700
> From: "Laurie Colbert" <
> Subject: [Histonet] Specimen Release
> To: <
> Message-ID:
> 	<
> 	
> Content-Type: text/plain;	charset="us-ascii"
> 
> Does anyone have a procedure they would be willing to share with me
> regarding the release of specimens to the patient?  We are revising our
> whole process and I'm interested in what others are doing.
> 
> 
> 
> Laurie Colbert
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Fri, 7 Aug 2009 10:21:11 -0700 (PDT)
> From: "Judith L. Williams" <
> Subject: [Histonet] Good Fibrin stain with Movat's?
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<
> Content-Type: TEXT/PLAIN; charset=US-ASCII; format=flowed
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> 
> Greetings to all histonetters- I have a pathologist that wants to show fibrin in lung. She wants also black nuclei and we tried  a Movat's to look at fibrin. The fibrin in the lung is showing up light blue not red!
> Any suggestions on how to show fibrin distinctly, with black nuclei?
> thanks!
> Judy
> 
> 
> 
> Judith Williams, PhD, HT(ASCP)
> Research Scientist
> Department of Comparative Medicine
> University of Washington
> Seattle, WA 98195
> 
> 
> 
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 7 Aug 2009 13:43:20 -0400
> From: thomas.crowell <@t> novartis.com
> Subject: [Histonet] Thomas Crowell is out of the office.
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
> 	<
> 	
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> 
> 
> I will be out of the office starting  08/07/2009 and will not return until
> 08/10/2009.
> 
> Please contact Humphrey Gardner at 617-871-3590 if you have any questions
> regarding clinical trial samples.
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 07 Aug 2009 14:35:23 -0400
> From: "Richard Cartun" <
> Subject: Re: [Histonet] Specimen Release
> To: "Laurie Colbert" <,
> 	<
> Message-ID: <
> Content-Type: text/plain; charset=US-ASCII
> 
> We no longer release specimens to patients.  If they want their tissue they have to go through a funeral home.  Here in CT, funeral homes are the only agencies licenced to bury or incinerate human tissue.
> 
> Richard
> 
> Richard W. Cartun, Ph.D.
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 545-1596
> (860) 545-0174 Fax
> 
> >>> "Laurie Colbert" < 8/7/2009 1:17 PM >>>
> Does anyone have a procedure they would be willing to share with me
> regarding the release of specimens to the patient?  We are revising our
> whole process and I'm interested in what others are doing.
> 
> 
> 
> Laurie Colbert
> 
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> 
> 
> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 7 Aug 2009 14:06:28 -0500
> From: "Vicki Kalscheur" <
> Subject: [Histonet] Peggy-cement in bone
> To: "Histonet Discussion" <
> Message-ID: <
> Content-Type: text/plain;	charset="iso-8859-1"
> 
> Hi Peggy,  From my experience, during the decal process the cement will fall out or loosen enough so that you can gently remove it and maintain fibrous ingrowth and detail. I have removed ceramic; implants; stents and cement using a scalpel and then embedded in paraffin for cuts on a sledge microtome. The detail is beautiful even without the cement present. If you have the capability, a corresponding section in PMMA can be processed.  ~ Vicki
> 
> ------------------------------
> 
> Message: 6
> Date: Fri, 07 Aug 2009 15:12:52 -0400
> From: Yash Kolambkar <
> Subject: [Histonet] Stain for decalcified GMA embedded bone
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
> 
> Hi,
> 
> I am looking for a stain for formalin fixed, decalcified (2 weeks in 
> CalEx II), GMA embedded rat femur samples that still has some muscle as 
> well as fibrous tissue. I would like the stain to distinguish the bone 
> from the muscle and fibrous tissue. I do not need to study the 
> microstructure of the bone, just need something that can contrast the 
> different tissues.
> 
> I've tried the standard Masson's trichrome (with Aniline Blue), but the 
> bone stained red and the contrast between the bone and the rest of the 
> tissue is not strong. Same with H&E.
> 
> Please let me know if you have any experience with such a stain. Any 
> help would be much appreciated.
> 
> Thanks!
> 
> Yash
> -- 
> Yash Kolambkar
> Graduate Research Assistant
> Department of Biomedical Engineering
> Georgia Tech/Emory Center for the Engineering of Living Tissues
> yashk <@t> gatech.edu
> 
> 
> 
> ------------------------------
> 
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> End of Histonet Digest, Vol 69, Issue 8
> ***************************************