[Histonet] CD31 working protocol

[GT Hebert]

Hi Patricia,
 
What tissue (animal / human) are you using?  How has it been fixed /  processed?
 
If you are using 10%NBF fixed, paraffin-embedded mouse tissue, you can use Biocare's CD31 with a simple indirect protocol.  I come in with my primary (overnight at 5ug/ml) after Prot. K digestion (DAKO ready-to-use), and then come in with a secondary Alexa fluor 488 against my primary.
 
It work really well.  I have also used it for frozen sections.
 
I hope this helps.
 
Regards.
 
Gustave
 
Gustave T. Hebert
Research Scientist I
Metabolic Disease Research
Wyeth Research
200 CambridgePark Drive
Cambridge, MA 02140

--- On Mon, 8/10/09, Zerfas, Patricia (NIH/OD/ORS) [E] <zerfasp <@t> ors.od.nih.gov> wrote:


From: Zerfas, Patricia (NIH/OD/ORS) [E] <zerfasp <@t> ors.od.nih.gov>
Subject: [Histonet] CD31 working protocol
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu>
Date: Monday, August 10, 2009, 2:50 PM


Dear Listservers,
            I have tried MANY protocols and I am unable to obtain a working protocol for the CD31 antibody.
            I have tried both the CD31 rat monoclonal and a CD31 rabbit polyclonal.

Thanks in advance for your help.

Patricia Zerfas
National Institutes of Health
Building 28A, Room 112
28 Library Drive
Bethesda, MD  20892
ph:   (301) 496-4464
fax:  (301) 402-1068

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