[Histonet] Stain for decalcified GMA embedded bone

Yash Kolambkar yashk <@t> gatech.edu
Fri Aug 7 14:12:52 CDT 2009


I am looking for a stain for formalin fixed, decalcified (2 weeks in 
CalEx II), GMA embedded rat femur samples that still has some muscle as 
well as fibrous tissue. I would like the stain to distinguish the bone 
from the muscle and fibrous tissue. I do not need to study the 
microstructure of the bone, just need something that can contrast the 
different tissues.

I've tried the standard Masson's trichrome (with Aniline Blue), but the 
bone stained red and the contrast between the bone and the rest of the 
tissue is not strong. Same with H&E.

Please let me know if you have any experience with such a stain. Any 
help would be much appreciated.


Yash Kolambkar
Graduate Research Assistant
Department of Biomedical Engineering
Georgia Tech/Emory Center for the Engineering of Living Tissues
yashk <@t> gatech.edu

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